Expression of medial class I histocompatibility antigens on RMA-S mutant cells

Evan Hermel, Elena Grigorenko, Kirsten Fischer Lindahl

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The RMA-S mutant T cell line is defective in H-2b restricted antigen presentation and has markedly reduced surface expression of Kb and Db. We examined RMA-S for the expression of the medial class I histocompatibility antigens Qa1b and Mta. While RMA-S targets varied in their susceptibility to lysis by cytotoxic T lymphocytes (CTL) specific for Qa1b, Mta levels were detectable but consistently low compared to the parent RMA cell line. Addition of synthetic ND1α1 - 26 or ND1α1 - 17 peptides that mimic MTFα (the ligand of Mta) increased killing of RMA-S by anti-Mtaα CTL to levels comparable to or better than RMA, with 300 nM peptide being fully effective. None of the MTF peptides increased the killing of RMA-S by anti-H-2b or anti-Qa1b CTL, even at the highest (1 μM) peptide concentrations. RMA-S cells treated with 100 μM of either the ND1α4 - 26 or ND1α1 - 26 peptides showed a small increase in the fluorescent staining for β2-microglobulin but not for H-2Kb or H-2Db. These results show that Mta and Qa1b, although affected, are not obliterated by the defect in RMA-S cells; that the association of MTF peptides with HMT is exclusive; and that MTF enters the endoplasmic reticulum in the same fashion as other endogenous peptides.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalInternational Immunology
Volume3
Issue number4
DOIs
StatePublished - Apr 1991

Keywords

  • Antigen presentation
  • Cytotoxic T lymphocytes
  • Mitochondria
  • Mta, Qa1

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation
  • Immunology

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