Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae

Bing Li, T. Li, X. Tong, D. Wang, J. Zhao, G. Wu

Research output: Contribution to journalArticle

Abstract

The psaC Gene of Synechococcus sp. PCC 7002 encodes the F A /F B aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae. Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

Original languageEnglish (US)
Pages (from-to)755-759
Number of pages5
JournalBeijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum Naturalium Universitatis Pekinensis
Volume37
Issue number6
StatePublished - Nov 1 2001
Externally publishedYes

Fingerprint

Cyanobacteria
Saccharomyces cerevisiae
Gene Expression
Genes
Synechococcus
Photosystem I Protein Complex
Southern Blotting
Molecular Weight
Proteins

Keywords

  • Cyanobacteria
  • psaC
  • Saccharomyces cerevisiae
  • Transformation

ASJC Scopus subject areas

  • General

Cite this

Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae. / Li, Bing; Li, T.; Tong, X.; Wang, D.; Zhao, J.; Wu, G.

In: Beijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum Naturalium Universitatis Pekinensis, Vol. 37, No. 6, 01.11.2001, p. 755-759.

Research output: Contribution to journalArticle

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AU - Li, T.

AU - Tong, X.

AU - Wang, D.

AU - Zhao, J.

AU - Wu, G.

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