Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae

B. Li; T. Li; X. Tong; D. Wang; J. Zhao; G. Wu

Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae

B. Li, T. Li, X. Tong, D. Wang, J. Zhao, G. Wu

Research output: Contribution to journal › Article › peer-review

Abstract

The psaC Gene of Synechococcus sp. PCC 7002 encodes the F_A/F_B aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae. Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

Original language	English (US)
Pages (from-to)	755-759
Number of pages	5
Journal	Beijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum Naturalium Universitatis Pekinensis
Volume	37
Issue number	6
State	Published - Nov 2001
Externally published	Yes

Keywords

Cyanobacteria
Saccharomyces cerevisiae
Transformation
psaC

ASJC Scopus subject areas

General

Cite this

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title = "Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae",

abstract = "The psaC Gene of Synechococcus sp. PCC 7002 encodes the FA/FB aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae. Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.",

keywords = "Cyanobacteria, Saccharomyces cerevisiae, Transformation, psaC",

author = "B. Li and T. Li and X. Tong and D. Wang and J. Zhao and G. Wu",

year = "2001",

month = nov,

language = "English (US)",

volume = "37",

pages = "755--759",

journal = "Beijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum Naturalium Universitatis Pekinensis",

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publisher = "Peking University",

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T1 - Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae

AU - Li, B.

AU - Li, T.

AU - Tong, X.

AU - Wang, D.

AU - Zhao, J.

AU - Wu, G.

PY - 2001/11

Y1 - 2001/11

N2 - The psaC Gene of Synechococcus sp. PCC 7002 encodes the FA/FB aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae. Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

AB - The psaC Gene of Synechococcus sp. PCC 7002 encodes the FA/FB aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae. Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

KW - Cyanobacteria

KW - Saccharomyces cerevisiae

KW - Transformation

KW - psaC

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M3 - Article

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EP - 759

JO - Beijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum Naturalium Universitatis Pekinensis

JF - Beijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum Naturalium Universitatis Pekinensis

IS - 6

ER -

Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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