Expression of photosynthetic gene psaC of cyanobacteria in Saccharomyces cerevisiae

B. Li, T. Li, X. Tong, D. Wang, J. Zhao, G. Wu

Research output: Contribution to journalArticle

Abstract

The psaC Gene of Synechococcus sp. PCC 7002 encodes the FA/FB aprotein of photosystem I, a protein with a molecular weight of 8.9 kD containing two 4Fe-4S centers. The expression vector of psaC gene was constructed, and transformed into Saccharoromyces cerevisiae. Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture.

Original languageEnglish (US)
Pages (from-to)755-759
Number of pages5
JournalBeijing Daxue Xuebao Ziran Kexue Ban/Acta Scientiarum Naturalium Universitatis Pekinensis
Volume37
Issue number6
StatePublished - Nov 1 2001
Externally publishedYes

Keywords

  • Cyanobacteria
  • Saccharomyces cerevisiae
  • Transformation
  • psaC

ASJC Scopus subject areas

  • General

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