Expression of the E. coli Lac Z gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells

David A. Boothman, Alfred I. Geller, Arthur B. Pardee

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Introducing foreign genetic material into human cells is essential for the elucidation of the function of various human genes and has potential use in the treatment of human diseases by gene therapy. In this study we demonstrate that a defective herpes simplex virus type 1 vector, pHSVlac, can effectively transfer and express the Escherichia coli Lac Z gene in a variety of exponential and quiescent human cells. The human cells tested included representative cells derived from cancer-prone patients that presumably have various DNA repair deficiencies.

Original languageEnglish (US)
Pages (from-to)159-162
Number of pages4
JournalFEBS Letters
Volume258
Issue number1
DOIs
StatePublished - Nov 20 1989

Keywords

  • DNA damage, potentially lethal
  • DNA repair
  • Gene therapy
  • HSV-1 vector, defective
  • Host range

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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