Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells

Hiroaki Kusanagi, Akiko Hida, Kohtoku Satoh, Masaru Echizenya, Tetsuo Shimizu, Julie S. Pendergast, Shin Yamazaki, Kazuo Mishima

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalNeuroscience Research
Volume61
Issue number2
DOIs
Publication statusPublished - Jun 2008

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Keywords

  • Circadian
  • Clock genes
  • Expression profiles
  • Human peripheral blood mononuclear cells (PBMCs)
  • Period (Per)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kusanagi, H., Hida, A., Satoh, K., Echizenya, M., Shimizu, T., Pendergast, J. S., ... Mishima, K. (2008). Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells. Neuroscience Research, 61(2), 136-142. https://doi.org/10.1016/j.neures.2008.01.012