FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c

Krishna Kumar Vasudevan, Kangkang Song, Lea M. Alford, Winfield S. Sale, Erin E. Dymek, Elizabeth F. Smith, Todd Hennessey, Ewa Joachimiak, Paulina Urbanska, Dorota Wloga, William Dentler, Daniela Nicastro, Jacek Gaertig

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Radial spokes are conserved macromolecular complexes that are essential for ciliary motility. A triplet of three radial spokes, RS1, RS2, and RS3, repeats every 96 nm along the doublet microtubules. Each spoke has a distinct base that docks to the doublet and is linked to different inner dynein arms. Little is known about the assembly and functions of individual radial spokes. A knockout of the conserved ciliary protein FAP206 in the ciliate Tetrahymena resulted in slow cell motility. Cryo-electron tomography showed that in the absence of FAP206, the 96-nm repeats lacked RS2 and dynein c. Occasionally, RS2 assembled but lacked both the front prong of its microtubule base and dynein c, whose tail is attached to the front prong. Overexpressed GFP-FAP206 decorated nonciliary microtubules in vivo. Thus FAP206 is likely part of the front prong and docks RS2 and dynein c to the microtubule.

Original languageEnglish (US)
Pages (from-to)696-710
Number of pages15
JournalMolecular Biology of the Cell
Volume26
Issue number4
DOIs
StatePublished - Jan 1 2015

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Dyneins
Microtubules
Electron Microscope Tomography
Macromolecular Substances
Tetrahymena
Cell Movement
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Vasudevan, K. K., Song, K., Alford, L. M., Sale, W. S., Dymek, E. E., Smith, E. F., ... Gaertig, J. (2015). FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c. Molecular Biology of the Cell, 26(4), 696-710. https://doi.org/10.1091/mbc.E14-11-1506

FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c. / Vasudevan, Krishna Kumar; Song, Kangkang; Alford, Lea M.; Sale, Winfield S.; Dymek, Erin E.; Smith, Elizabeth F.; Hennessey, Todd; Joachimiak, Ewa; Urbanska, Paulina; Wloga, Dorota; Dentler, William; Nicastro, Daniela; Gaertig, Jacek.

In: Molecular Biology of the Cell, Vol. 26, No. 4, 01.01.2015, p. 696-710.

Research output: Contribution to journalArticle

Vasudevan, KK, Song, K, Alford, LM, Sale, WS, Dymek, EE, Smith, EF, Hennessey, T, Joachimiak, E, Urbanska, P, Wloga, D, Dentler, W, Nicastro, D & Gaertig, J 2015, 'FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c', Molecular Biology of the Cell, vol. 26, no. 4, pp. 696-710. https://doi.org/10.1091/mbc.E14-11-1506
Vasudevan KK, Song K, Alford LM, Sale WS, Dymek EE, Smith EF et al. FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c. Molecular Biology of the Cell. 2015 Jan 1;26(4):696-710. https://doi.org/10.1091/mbc.E14-11-1506
Vasudevan, Krishna Kumar ; Song, Kangkang ; Alford, Lea M. ; Sale, Winfield S. ; Dymek, Erin E. ; Smith, Elizabeth F. ; Hennessey, Todd ; Joachimiak, Ewa ; Urbanska, Paulina ; Wloga, Dorota ; Dentler, William ; Nicastro, Daniela ; Gaertig, Jacek. / FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c. In: Molecular Biology of the Cell. 2015 ; Vol. 26, No. 4. pp. 696-710.
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