Fetal mouse hearts: a model for studying ischemia

J. S. Ingwall, M. DeLuca, H. D. Sybers, K. Wildenthal

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A new experimental model for the study of two important aspects of ischemia, namely, oxygen and substrate deprivation, is proposed: the intact, beating fetal mouse heart in organ culture. This model offers long term stability, ease and reproducibility of preparation, and the ability to manipulate experimental conditions. Hearts deprived of oxygen and glucose ceased beating immediately. After 3-4 hr of deprivation, biochemical and ultrastructural changes consistent with ischemic injury were evident. These include depletion of ATP and glycogen levels, loss of cytoplasmic enzymes, and extensive swelling and disruption of mitochondrial structure. Glucose and insulin partially protected against ATP depletion. Upon resupply of oxygen and glucose, beating resumed immediately, ATP levels rapidly increased to control levels and, consistent with this, mitochondrial structure returned toward normal. During the recovery phase autophagic vacuoles containing damaged mitochondria and myofibrils were seen, indicating that repair mechanisms were activated. Consistent with this, the proportion of lysosomal enzymes that were present in the nonsedimentable fraction of the tissue homogenate increased. It is concluded that the cultured fetal mouse heart is a model useful for studying myocardial responses to anoxia and/or substrate deprivation and for assessing interventions designed to limit damage or to stimulate repair after ischemic injury.

Original languageEnglish (US)
Pages (from-to)2809-2813
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number7
StatePublished - 1975

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Fetal Heart
Ischemia
Adenosine Triphosphate
Oxygen
Glucose
Mitochondrial Swelling
Myofibrils
Organ Culture Techniques
Wounds and Injuries
Enzymes
Vacuoles
Glycogen
Mitochondria
Theoretical Models
Insulin

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Ingwall, J. S., DeLuca, M., Sybers, H. D., & Wildenthal, K. (1975). Fetal mouse hearts: a model for studying ischemia. Proceedings of the National Academy of Sciences of the United States of America, 72(7), 2809-2813.

Fetal mouse hearts : a model for studying ischemia. / Ingwall, J. S.; DeLuca, M.; Sybers, H. D.; Wildenthal, K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, No. 7, 1975, p. 2809-2813.

Research output: Contribution to journalArticle

Ingwall, JS, DeLuca, M, Sybers, HD & Wildenthal, K 1975, 'Fetal mouse hearts: a model for studying ischemia', Proceedings of the National Academy of Sciences of the United States of America, vol. 72, no. 7, pp. 2809-2813.
Ingwall, J. S. ; DeLuca, M. ; Sybers, H. D. ; Wildenthal, K. / Fetal mouse hearts : a model for studying ischemia. In: Proceedings of the National Academy of Sciences of the United States of America. 1975 ; Vol. 72, No. 7. pp. 2809-2813.
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