Fibroblast growth factors modulate intestinal epithelial cell growth and migration

Axel U. Dignass, Shoji Tsunekawa, Daniel K. Podolsky

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

Background/Aims: Various peptide growth factors have been found to exert functional effects among epithelial cell populations. This study assessed the role of certain fibroblast growth factors (FGFs) (acidic FGF, basic FGF, and keratinocyte growth factor) in the regulation of intestinal epithelial cell proliferation and restitution. Methods: Recombinant growth factors were added to subconfluent cultures of IEC-6, Caco-2, and HT-29 cell lines with subsequent assessment of [3H]-thymidine incorporation. The effects on an in vitro model of restitution were assessed by quantitation of cells migrating into standard wounds established in confluent monolayers of IEC-6 cells. Transforming growth factor β (TGF-β) content of growth factortreated wounded monolayers was assessed by Northern blot and bioassay. Results: Acidic FGF, basic FGF, and keratinocyte growth factor caused a modest increase in proliferation of IEC-6, Caco-2, and HT-29 cell lines. Acidic FGF and basic FGF promoted intestinal epithelial cell restitution in vitro up to 10-fold, in conjunction with the enhanced expression of TGF-β messenger RNA and protein. Promotion of IEC-6 restitution by acidic and basic FGF could be blocked by addition of immunoneutralizing anti-TGF-β antisera. Conclusions: FGFs that exert effects on fibroblast cells also exert effects on intestinal epithelial cell populations and may help promote epithelial cell restitution, the initial step of intestinal wound healing through a TGF-β-dependent pathway.

Original languageEnglish (US)
Pages (from-to)1254-1262
Number of pages9
JournalGastroenterology
Volume106
Issue number5
StatePublished - 1994

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Fibroblast Growth Factors
Fibroblast Growth Factor 1
Cell Movement
Transforming Growth Factors
Fibroblast Growth Factor 2
Epithelial Cells
Fibroblast Growth Factor 7
Growth
HT29 Cells
Intercellular Signaling Peptides and Proteins
Cell Line
Biological Assay
Northern Blotting
Wound Healing
Thymidine
Population
Immune Sera
Fibroblasts
Cell Proliferation
Messenger RNA

ASJC Scopus subject areas

  • Gastroenterology

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Fibroblast growth factors modulate intestinal epithelial cell growth and migration. / Dignass, Axel U.; Tsunekawa, Shoji; Podolsky, Daniel K.

In: Gastroenterology, Vol. 106, No. 5, 1994, p. 1254-1262.

Research output: Contribution to journalArticle

Dignass, Axel U. ; Tsunekawa, Shoji ; Podolsky, Daniel K. / Fibroblast growth factors modulate intestinal epithelial cell growth and migration. In: Gastroenterology. 1994 ; Vol. 106, No. 5. pp. 1254-1262.
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