Fibroblast spreading and phagocytosis: Similar cell responses to different-sized substrata

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Experiments were carried out to test the hypothesis that cell spreading and phagocytosis are similar cell responses to different-sized substrata. The following morphological and biochemical studies provided evidence for this supposition. Cells phagocytosed to 1.09-μm and 5.7-μm latex beads, but were unable to completely ingest 15.8-μm or 25.7-μm beads. With the larger beads, the cells spread around the bead surfaces with an appearance typical of cells spread on culture dishes. Biochemical studies with cytochalasin D, azide, and iodoacetate, as well as temperature-dependence studies, demonstrated similar responses of cell spreading and phagocytosis to these treatments. Similar cell surface receptors were involved in cell spreading and phagocytosis based upon experiments using antibodies to baby hamster kidney (BHK) cell wheat germ agglutinin receptors. And finally, BHK cell variants with defective plasma fibronectin (pFN) receptors were unable to spread on pFN-coated dishes or ingest pFN-coated beads. Evidence also is presented concerning the 'contact' process in cell adhesion. It was found that azide and low temperature inhibited cell attachment per se but did not block fibronectin-receptor interactions based upon cell binding of pFN-coated beads. A possible explanation for the contact process is presented based upon the resistance of cells and beads to shear forces.

Original languageEnglish (US)
Pages (from-to)58-64
Number of pages7
JournalJournal of Cellular Physiology
Volume119
Issue number1
StatePublished - 1984

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Cytophagocytosis
Fibroblasts
Fibronectins
Plasmas
Azides
Fibronectin Receptors
Integrin alpha5beta1
Iodoacetates
Cytochalasin D
Cell adhesion
Latex
Cricetinae
Cell Surface Receptors
Beam plasma interactions
Kidney
Experiments
Temperature
Plasma Cells
Antibodies
Microspheres

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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Fibroblast spreading and phagocytosis : Similar cell responses to different-sized substrata. / Grinnell, F.

In: Journal of Cellular Physiology, Vol. 119, No. 1, 1984, p. 58-64.

Research output: Contribution to journalArticle

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