Fibronectin profiles in normal and chronic wound fluid

A. B. Wysocki, F. Grinnell

Research output: Contribution to journalArticle

108 Scopus citations

Abstract

Fibronectin, an adhesion protein found in blood and tissues, is an important factor in wound repair. Recently, we began studies using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting to compare fibronectin in the fluid of normal and chronic wounds. We found similar fibronectin profiles in suction blister fluid (2 normal volunteers) and mastectomy fluid (5 patients). There was primarily an intact 250 kilodalton fibronectin subunit and in addition some higher molecular mass fibronectin-containing complexes. On the other hand, fibronectin in diabetic ulcer wound fluid (2 patients) was partially degraded, and there was no intact fibronectin in stasis ulcer wound fluid (2 patients). The most prominent degradation products were 93 and 125 kilodalton polypeptides. When intact fibronectin was added back to stasis ulcer wound fluid, fragmentation occurred within 15 minutes at 37°C indicating the presence of protease activity in the wound fluid.

Original languageEnglish (US)
Pages (from-to)825-831
Number of pages7
JournalLaboratory Investigation
Volume63
Issue number6
StatePublished - Dec 1 1990

Keywords

  • Nonhealing ulcers
  • Proteolysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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