First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods

Dieter Lütjohann, Ingemar Björkhem, Silvia Friedrichs, Anja Kerksiek, Anita Lövgren-Sandblom, Wolf Jochen Geilenkeuser, Robert Ahrends, Isabel Andrade, Diana Ansorena, Iciar Astiasarán, Lucía Baila-Rueda, Bianca Barriuso, Susen Becker, Lionel Bretillon, Richard W. Browne, Claudio Caccia, Uta Ceglarek, Ana Cenarro, Peter J. Crick, Günter FaulerGuadalupe Garcia-Llatas, Robert Gray, William J. Griffiths, Helena Gylling, Scott Harding, Christin Helmschrodt, Luigi Iuliano, Hans Gerd Janssen, Peter Jones, Leena Kaipiainen, Frank Kannenberg, María Jesús Lagarda, Valerio Leoni, Ana Maria Lottenberg, Dylan S. MacKay, Silke Matysik, Jeff McDonald, Maria Menendez-Carreño, Semone B. Myrie, Valéria Sutti Nunes, Richard E. Ostlund, Eliana Polisecki, Fernando Ramos, Todd C. Rideout, Ernst J. Schaefer, Gerd Schmitz, Yuqin Wang, Chiara Zerbinati, Ulf Diczfalusy, Hans Frieder Schött

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys, each laboratory used its own internal standard (5α-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs.

Original languageEnglish (US)
Pages (from-to)115-125
Number of pages11
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume190
DOIs
StatePublished - Jun 2019

Keywords

  • Atherosclerosis
  • Cholesterol absorption
  • Cholesterol balance
  • Cholesterol synthesis
  • Phytosterols
  • Surrogate marker

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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  • Cite this

    Lütjohann, D., Björkhem, I., Friedrichs, S., Kerksiek, A., Lövgren-Sandblom, A., Geilenkeuser, W. J., Ahrends, R., Andrade, I., Ansorena, D., Astiasarán, I., Baila-Rueda, L., Barriuso, B., Becker, S., Bretillon, L., Browne, R. W., Caccia, C., Ceglarek, U., Cenarro, A., Crick, P. J., ... Schött, H. F. (2019). First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods. Journal of Steroid Biochemistry and Molecular Biology, 190, 115-125. https://doi.org/10.1016/j.jsbmb.2019.03.025