TY - JOUR
T1 - Fluorescence fluctuation spectroscopy
T2 - Ushering in a new age of enlightenment for cellular dynamics
AU - Jameson, David M.
AU - Ross, Justin A.
AU - Albanesi, Joseph P.
N1 - Funding Information:
Acknowledgments The authors wish to thank Nicholas James for critically reading the manuscript. This work was supported by National Institutes of Health grant RO1GM076665 (DMJ) and a grant from Allergan, Inc.
PY - 2009
Y1 - 2009
N2 - Originally developed for applications in physics and physical chemistry, fluorescence fluctuation spectroscopy is becoming widely used in cell biology. This review traces the development of the method and describes some of the more important applications. Specifically, the methods discussed include fluorescence correlation spectroscopy (FCS), scanning FCS, dual color cross-correlation FCS, the photon counting histogram and fluorescence intensity distribution analysis approaches, the raster scanning image correlation spectroscopy method, and the Number and Brightness technique. The physical principles underlying these approaches will be delineated, and each of the methods will be illustrated using examples from the literature.
AB - Originally developed for applications in physics and physical chemistry, fluorescence fluctuation spectroscopy is becoming widely used in cell biology. This review traces the development of the method and describes some of the more important applications. Specifically, the methods discussed include fluorescence correlation spectroscopy (FCS), scanning FCS, dual color cross-correlation FCS, the photon counting histogram and fluorescence intensity distribution analysis approaches, the raster scanning image correlation spectroscopy method, and the Number and Brightness technique. The physical principles underlying these approaches will be delineated, and each of the methods will be illustrated using examples from the literature.
KW - Fluorescence correlation spectroscopy
KW - Fluorescence fluctuation spectroscopy
KW - Number and brightness technique
KW - Photon counting histogram
KW - Raster scanning image correlation spectroscopy
KW - Scanning FCS
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U2 - 10.1007/s12551-009-0013-8
DO - 10.1007/s12551-009-0013-8
M3 - Review article
C2 - 21547245
AN - SCOPUS:77952485825
SN - 1867-2450
VL - 1
SP - 105
EP - 118
JO - Biophysical Reviews
JF - Biophysical Reviews
IS - 3
ER -