Fluorescence fluctuation spectroscopy: Ushering in a new age of enlightenment for cellular dynamics

David M. Jameson, Justin A. Ross, Joseph P. Albanesi

Research output: Contribution to journalReview article

31 Scopus citations

Abstract

Originally developed for applications in physics and physical chemistry, fluorescence fluctuation spectroscopy is becoming widely used in cell biology. This review traces the development of the method and describes some of the more important applications. Specifically, the methods discussed include fluorescence correlation spectroscopy (FCS), scanning FCS, dual color cross-correlation FCS, the photon counting histogram and fluorescence intensity distribution analysis approaches, the raster scanning image correlation spectroscopy method, and the Number and Brightness technique. The physical principles underlying these approaches will be delineated, and each of the methods will be illustrated using examples from the literature.

Original languageEnglish (US)
Pages (from-to)105-118
Number of pages14
JournalBiophysical Reviews
Volume1
Issue number3
DOIs
StatePublished - 2009

Keywords

  • Fluorescence correlation spectroscopy
  • Fluorescence fluctuation spectroscopy
  • Number and brightness technique
  • Photon counting histogram
  • Raster scanning image correlation spectroscopy
  • Scanning FCS

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology

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