Fluorescent modification of adenosine 3′,5′-monophosphate

Spectroscopic properties and activity in enzyme systems

John A. Secrist, Jorge R. Barrio, Nelson J. Leonard, Carlos Villar-Palasi, Alfred G. Gilman

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N6-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-nonophosphate. The potential utility of this analog is indicated by its long flouorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.

Original languageEnglish (US)
Pages (from-to)279-280
Number of pages2
JournalScience
Volume177
Issue number4045
StatePublished - 1972

Fingerprint

Adenosine
Enzymes
Protein Kinases

ASJC Scopus subject areas

  • General

Cite this

Secrist, J. A., Barrio, J. R., Leonard, N. J., Villar-Palasi, C., & Gilman, A. G. (1972). Fluorescent modification of adenosine 3′,5′-monophosphate: Spectroscopic properties and activity in enzyme systems. Science, 177(4045), 279-280.

Fluorescent modification of adenosine 3′,5′-monophosphate : Spectroscopic properties and activity in enzyme systems. / Secrist, John A.; Barrio, Jorge R.; Leonard, Nelson J.; Villar-Palasi, Carlos; Gilman, Alfred G.

In: Science, Vol. 177, No. 4045, 1972, p. 279-280.

Research output: Contribution to journalArticle

Secrist, JA, Barrio, JR, Leonard, NJ, Villar-Palasi, C & Gilman, AG 1972, 'Fluorescent modification of adenosine 3′,5′-monophosphate: Spectroscopic properties and activity in enzyme systems', Science, vol. 177, no. 4045, pp. 279-280.
Secrist JA, Barrio JR, Leonard NJ, Villar-Palasi C, Gilman AG. Fluorescent modification of adenosine 3′,5′-monophosphate: Spectroscopic properties and activity in enzyme systems. Science. 1972;177(4045):279-280.
Secrist, John A. ; Barrio, Jorge R. ; Leonard, Nelson J. ; Villar-Palasi, Carlos ; Gilman, Alfred G. / Fluorescent modification of adenosine 3′,5′-monophosphate : Spectroscopic properties and activity in enzyme systems. In: Science. 1972 ; Vol. 177, No. 4045. pp. 279-280.
@article{77d627c89b6645228044b01313b6595f,
title = "Fluorescent modification of adenosine 3′,5′-monophosphate: Spectroscopic properties and activity in enzyme systems",
abstract = "The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N6-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-nonophosphate. The potential utility of this analog is indicated by its long flouorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.",
author = "Secrist, {John A.} and Barrio, {Jorge R.} and Leonard, {Nelson J.} and Carlos Villar-Palasi and Gilman, {Alfred G.}",
year = "1972",
language = "English (US)",
volume = "177",
pages = "279--280",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4045",

}

TY - JOUR

T1 - Fluorescent modification of adenosine 3′,5′-monophosphate

T2 - Spectroscopic properties and activity in enzyme systems

AU - Secrist, John A.

AU - Barrio, Jorge R.

AU - Leonard, Nelson J.

AU - Villar-Palasi, Carlos

AU - Gilman, Alfred G.

PY - 1972

Y1 - 1972

N2 - The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N6-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-nonophosphate. The potential utility of this analog is indicated by its long flouorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.

AB - The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N6-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-nonophosphate. The potential utility of this analog is indicated by its long flouorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.

UR - http://www.scopus.com/inward/record.url?scp=0015518517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015518517&partnerID=8YFLogxK

M3 - Article

VL - 177

SP - 279

EP - 280

JO - Science

JF - Science

SN - 0036-8075

IS - 4045

ER -