Abstract
We have used polyclonal anti-synthetic peptide serum to study the role of retinoblastoma gene (RB) innctivation in a variety of human tumor cell lines. Our analysis indicates that inactivation of the RB protein, p105-Rb, is universal in retinoblastoma cells, vindicating the predictions of the Knudson "two-hit" hypothesis. In addition, our analysis has shown that inactivations of the RB gene are nearly as frequent in a more common human tumor, small cell lung carcinoma. One-third of bladder carcinomas surveyed also carry altered or absent p105-Rb. Other human tumors by contrast demonstrate only infrequent inactivation of the RB gene. These results suggest that inactivation of the RB gene is a critical step in the pathogenesis of a subset of human tumors.
Original language | English (US) |
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Pages (from-to) | 2775-2779 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 87 |
Issue number | 7 |
State | Published - Apr 1990 |
Keywords
- Recessive oncogene
- Retinoblastoma protein
- Splicing
ASJC Scopus subject areas
- General