From particle tracking to molecular interactions

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Live cell imaging often requires single particle tracking to capture the spatio-temporal details of molecular dynamics in situ. Here we present a single-particle tracking algorithm that constructs complete and unbiased trajectories from time-lapse sequences: It first links detected particles frame-by-frame, then closes gaps between initial tracks, accounting for particle appearance, disappearance, merging and splitting. Both steps are compactly formulated as a linear assignment problem, leading to a globally optimal solution. Tracking is supported by on-the-fly classification of particle motion. The tracking algorithm is flexible and versatile. We have applied it to study trans-membrane protein interactions, endocytic pit lifetime distributions and sister kinetochore motion and coupling.

Original languageEnglish (US)
Title of host publication2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI
Pages839-842
Number of pages4
DOIs
StatePublished - 2008
Event2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI - Paris, France
Duration: May 14 2008May 17 2008

Other

Other2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI
CountryFrance
CityParis
Period5/14/085/17/08

Fingerprint

Molecular interactions
Merging
Molecular dynamics
Trajectories
Proteins
Membranes
Imaging techniques

Keywords

  • Gap closing
  • Occlusion
  • Single molecule imaging
  • Single particle tracking

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Jaqaman, K. (2008). From particle tracking to molecular interactions. In 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI (pp. 839-842). [4541127] https://doi.org/10.1109/ISBI.2008.4541127

From particle tracking to molecular interactions. / Jaqaman, Khuloud.

2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI. 2008. p. 839-842 4541127.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jaqaman, K 2008, From particle tracking to molecular interactions. in 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI., 4541127, pp. 839-842, 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI, Paris, France, 5/14/08. https://doi.org/10.1109/ISBI.2008.4541127
Jaqaman K. From particle tracking to molecular interactions. In 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI. 2008. p. 839-842. 4541127 https://doi.org/10.1109/ISBI.2008.4541127
Jaqaman, Khuloud. / From particle tracking to molecular interactions. 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI. 2008. pp. 839-842
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