From particle tracking to molecular interactions

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Live cell imaging often requires single particle tracking to capture the spatio-temporal details of molecular dynamics in situ. Here we present a single-particle tracking algorithm that constructs complete and unbiased trajectories from time-lapse sequences: It first links detected particles frame-by-frame, then closes gaps between initial tracks, accounting for particle appearance, disappearance, merging and splitting. Both steps are compactly formulated as a linear assignment problem, leading to a globally optimal solution. Tracking is supported by on-the-fly classification of particle motion. The tracking algorithm is flexible and versatile. We have applied it to study trans-membrane protein interactions, endocytic pit lifetime distributions and sister kinetochore motion and coupling.

Original languageEnglish (US)
Title of host publication2008 5th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, Proceedings, ISBI
Pages839-842
Number of pages4
DOIs
StatePublished - Sep 10 2008
Event2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI - Paris, France
Duration: May 14 2008May 17 2008

Publication series

Name2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI

Other

Other2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI
CountryFrance
CityParis
Period5/14/085/17/08

Keywords

  • Gap closing
  • Occlusion
  • Single molecule imaging
  • Single particle tracking

ASJC Scopus subject areas

  • Biomedical Engineering

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  • Cite this

    Jaqaman, K. (2008). From particle tracking to molecular interactions. In 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI (pp. 839-842). [4541127] (2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Proceedings, ISBI). https://doi.org/10.1109/ISBI.2008.4541127