FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora

Xiao Liu, Ahai Chen, Angélica Caicedo-Casso, Guofei Cui, Mingjian Du, Qun He, Sookkyung Lim, Hang J. Kim, Christian I. Hong, Yi Liu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Circadian clock mechanisms have been extensively investigated but the main rate-limiting step that determines circadian period remains unclear. Formation of a stable complex between clock proteins and CK1 is a conserved feature in eukaryotic circadian mechanisms. Here we show that the FRQ-CK1 interaction, but not FRQ stability, correlates with circadian period in Neurospora circadian clock mutants. Mutations that specifically affect the FRQ-CK1 interaction lead to severe alterations in circadian period. The FRQ-CK1 interaction has two roles in the circadian negative feedback loop. First, it determines the FRQ phosphorylation profile, which regulates FRQ stability and also feeds back to either promote or reduce the interaction itself. Second, it determines the efficiency of circadian negative feedback process by mediating FRQ-dependent WC phosphorylation. Our conclusions are further supported by mathematical modeling and in silico experiments. Together, these results suggest that the FRQ-CK1 interaction is a major rate-limiting step in circadian period determination.

Original languageEnglish (US)
Article number4352
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

neurospora
circadian rhythms
Neurospora
Circadian Clocks
Circadian Rhythm
Clocks
Phosphorylation
clocks
phosphorylation
negative feedback
Computer Simulation
Feedback
interactions
Mutation
mutations
Proteins
proteins
Experiments
profiles

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora. / Liu, Xiao; Chen, Ahai; Caicedo-Casso, Angélica; Cui, Guofei; Du, Mingjian; He, Qun; Lim, Sookkyung; Kim, Hang J.; Hong, Christian I.; Liu, Yi.

In: Nature communications, Vol. 10, No. 1, 4352, 01.12.2019.

Research output: Contribution to journalArticle

Liu, X, Chen, A, Caicedo-Casso, A, Cui, G, Du, M, He, Q, Lim, S, Kim, HJ, Hong, CI & Liu, Y 2019, 'FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora', Nature communications, vol. 10, no. 1, 4352. https://doi.org/10.1038/s41467-019-12239-w
Liu, Xiao ; Chen, Ahai ; Caicedo-Casso, Angélica ; Cui, Guofei ; Du, Mingjian ; He, Qun ; Lim, Sookkyung ; Kim, Hang J. ; Hong, Christian I. ; Liu, Yi. / FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora. In: Nature communications. 2019 ; Vol. 10, No. 1.
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