Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor

Roland Schüle, Pundl Rangarajan, Steven Kliewer, Lynn J. Ransone, Jack Bolado, Na Yang, Inder M. Verma, Ronald M. Evans

Research output: Contribution to journalArticle

988 Citations (Scopus)

Abstract

We present evidence that the glucocorticoid receptor (GR) and transcription factor Jun/AP-1 can reciprocally repress one another's transcriptional activation by a novel mechanism that is Independent of DNA binding. Overexpression of c-Jun prevents the glucocorticoid-induced activation of genes carrying a functional glucocorticoid response element (GRE). Conversely, GR is able to repress AP-1-mediated transcriptional activation. Mutant analysis reveals that the ligand binding and DNA binding domains of GR and the region including the leucine zipper of c-Jun are required for repression. Gel retardation analysis demonstrates that bacterially expressed c-Jun disrupts GR-GRE complexes. These data Indicate that members of two distinct classes of transcription factors can oppose one another's activity through a mechanism likely involving protein-protein interactions.

Original languageEnglish (US)
Pages (from-to)1217-1226
Number of pages10
JournalCell
Volume62
Issue number6
DOIs
StatePublished - Sep 21 1990

Fingerprint

Oncogene Proteins
Glucocorticoid Receptors
Transcriptional Activation
Glucocorticoids
Chemical activation
Transcription Factor AP-1
Response Elements
Transcription Factors
Leucine Zippers
DNA
Proteins
Genes
Gels
Ligands

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Schüle, R., Rangarajan, P., Kliewer, S., Ransone, L. J., Bolado, J., Yang, N., ... Evans, R. M. (1990). Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor. Cell, 62(6), 1217-1226. https://doi.org/10.1016/0092-8674(90)90397-W

Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor. / Schüle, Roland; Rangarajan, Pundl; Kliewer, Steven; Ransone, Lynn J.; Bolado, Jack; Yang, Na; Verma, Inder M.; Evans, Ronald M.

In: Cell, Vol. 62, No. 6, 21.09.1990, p. 1217-1226.

Research output: Contribution to journalArticle

Schüle, R, Rangarajan, P, Kliewer, S, Ransone, LJ, Bolado, J, Yang, N, Verma, IM & Evans, RM 1990, 'Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor', Cell, vol. 62, no. 6, pp. 1217-1226. https://doi.org/10.1016/0092-8674(90)90397-W
Schüle R, Rangarajan P, Kliewer S, Ransone LJ, Bolado J, Yang N et al. Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor. Cell. 1990 Sep 21;62(6):1217-1226. https://doi.org/10.1016/0092-8674(90)90397-W
Schüle, Roland ; Rangarajan, Pundl ; Kliewer, Steven ; Ransone, Lynn J. ; Bolado, Jack ; Yang, Na ; Verma, Inder M. ; Evans, Ronald M. / Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor. In: Cell. 1990 ; Vol. 62, No. 6. pp. 1217-1226.
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