@article{760003ab4900480a9fbfcfeaebc41a61,
title = "Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor",
abstract = "We present evidence that the glucocorticoid receptor (GR) and transcription factor Jun/AP-1 can reciprocally repress one another's transcriptional activation by a novel mechanism that is Independent of DNA binding. Overexpression of c-Jun prevents the glucocorticoid-induced activation of genes carrying a functional glucocorticoid response element (GRE). Conversely, GR is able to repress AP-1-mediated transcriptional activation. Mutant analysis reveals that the ligand binding and DNA binding domains of GR and the region including the leucine zipper of c-Jun are required for repression. Gel retardation analysis demonstrates that bacterially expressed c-Jun disrupts GR-GRE complexes. These data Indicate that members of two distinct classes of transcription factors can oppose one another's activity through a mechanism likely involving protein-protein interactions.",
author = "Roland Sch{\"u}le and Pundl Rangarajan and Steven Kliewer and Ransone, {Lynn J.} and Jack Bolado and Na Yang and Verma, {Inder M.} and Evans, {Ronald M.}",
note = "Funding Information: We thank Dr. Peter Herrlich for plasmids and exchange of information prior to publication, and William W. Lamph, Stan Hollenberg, and Kazuhiko Umesono for plasmids. We also thank Ester Banayo and Kevin Murakami for expert assistance, the members of the Evans laboratory, especially Drs. Henry Sucov and Tony Oro, for discussion and critical reading of the manuscript, and Elaine Stevens for administrative assistance and help in the preparation of the manuscript. R. S. is supported by a postdoctoral fellowship of the Deutsche Forschungs-gemeinschaft. S. K. is a Fellow of the Jane Coffin Child8 Memorial Fund for Medical Research. L. R. is supported by an NIH postdoctoral training grant (CA 08585-01). R. M. E. is an Investigator of the Howard Hughes Medical Institute. This work was supported by the Howard Hughes Medical Institute, the National Institutes of Health, the American Cancer Association, and the Mathers Foundation. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1990",
month = sep,
day = "21",
doi = "10.1016/0092-8674(90)90397-W",
language = "English (US)",
volume = "62",
pages = "1217--1226",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}