Functional genomic characterization of neoblast-like stem cells in larval Schistosoma mansoni

Bo Wang, James J. Collins, Phillip A. Newmark

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Schistosomes infect hundreds of millions of people in the developing world. Transmission of these parasites relies on a stem cell-driven, clonal expansion of larvae inside a molluscan intermediate host. How this novel asexual reproductive strategy relates to current models of stem cell maintenance and germline specification is unclear. Here, we demonstrate that this proliferative larval cell population (germinal cells) shares some molecular signatures with stem cells from diverse organisms, in particular neoblasts of planarians (free-living relatives of schistosomes). We identify two distinct germinal cell lineages that differ in their proliferation kinetics and expression of a nanos ortholog. We show that a vasa/PL10 homolog is required for proliferation and maintenance of both populations, whereas argonaute2 and a fibroblast growth factor receptor-encoding gene are required only for nanos-negative cells. Our results suggest that an ancient stem cell-based developmental program may have enabled the evolution of the complex life cycle of parasitic flatworms.

Original languageEnglish (US)
Article numbere00768
JournaleLife
Volume2013
Issue number2
DOIs
StatePublished - Jul 30 2013

Fingerprint

Schistosoma mansoni
Stem cells
Stem Cells
Maintenance
Platyhelminths
Planarians
Fibroblast Growth Factor Receptors
Gene encoding
Cell Lineage
Life Cycle Stages
Population
Larva
Life cycle
Parasites
Cells
Specifications
Kinetics
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Functional genomic characterization of neoblast-like stem cells in larval Schistosoma mansoni. / Wang, Bo; Collins, James J.; Newmark, Phillip A.

In: eLife, Vol. 2013, No. 2, e00768, 30.07.2013.

Research output: Contribution to journalArticle

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