Functional significance of the interaction with Ku in DNA double-strand break recognition of XLF

Ken Ichi Yano, Keiko Morotomi-Yano, Kyung Jong Lee, David J. Chen

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Ku heterodimer is essential for the repair of DNA double-strand breaks (DSBs) by non-homologous end-joining (NHEJ). Ku recruits XLF, also known as Cernunnos, to DSBs. Here we report domain analyses of Ku-XLF interaction. The heterodimeric domain of Ku was found to be sufficient for the recruitment of XLF to DSBs and for the interaction of Ku with XLF. A small C-terminal deletion of XLF completely abolished recruitment of XLF to DSBs and Ku-XLF interaction. This deletion also led to marked reduction of XLF-XRCC4 interaction although the XRCC4-binding site on the XLF N-terminal domain remained intact. These results demonstrate the significance of Ku-XLF interaction in the molecular assembly of NHEJ factors.

Original languageEnglish (US)
Pages (from-to)841-846
Number of pages6
JournalFEBS Letters
Volume585
Issue number6
DOIs
StatePublished - Mar 23 2011

Keywords

  • Cernunnos
  • DNA double-strand break
  • Ku
  • Non-homologous end-joining
  • XLF

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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