Fusion surface structure, function, and dynamics of gamete fusogen HAP2

Juan Feng, Xianchi Dong, Jennifer Pinello, Jun Zhang, Chafen Lu, Roxana E. Iacob, John R. Engen, William J. Snell, Timothy A. Springer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

HAP2 is a class II gamete fusogen in many eukaryotic kingdoms. A crystal structure of Chlamydomonas HAP2 shows a trimeric fusion state. Domains D1, D2.1 and D2.2 line the 3-fold axis; D3 and a stem pack against the outer surface. Surprisingly, hydrogen-deuterium exchange shows that surfaces of D1, D2.2 and D3 closest to the 3-fold axis are more dynamic than exposed surfaces. Three fusion helices in the fusion loops of each monomer expose hydrophobic residues at the trimer apex that are splayed from the 3-fold axis, leaving a solvent-filled cavity between the fusion loops in each monomer. At the base of the two fusion loops, Arg185 docks in a carbonyl cage. Comparisons to other structures, dynamics, and the greater effect on Chlamydomonas gamete fusion of mutation of axis-proximal than axis-distal fusion helices suggest that the apical portion of each monomer could tilt toward the 3-fold axis with merger of the fusion helices into a common fusion surface.

Original languageEnglish (US)
JournaleLife
Volume7
DOIs
StatePublished - Oct 3 2018

Fingerprint

Chlamydomonas
Germ Cells
Surface structure
Fusion reactions
Deuterium
Hydrogen
Mutation
Monomers
Docks
Crystal structure

Keywords

  • biochemistry
  • chemical biology
  • chlamydomonas reinhardtii
  • dynamics
  • fusion loop
  • HAP2
  • molecular biophysics
  • structural biology

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Feng, J., Dong, X., Pinello, J., Zhang, J., Lu, C., Iacob, R. E., ... Springer, T. A. (2018). Fusion surface structure, function, and dynamics of gamete fusogen HAP2. eLife, 7. https://doi.org/10.7554/eLife.39772

Fusion surface structure, function, and dynamics of gamete fusogen HAP2. / Feng, Juan; Dong, Xianchi; Pinello, Jennifer; Zhang, Jun; Lu, Chafen; Iacob, Roxana E.; Engen, John R.; Snell, William J.; Springer, Timothy A.

In: eLife, Vol. 7, 03.10.2018.

Research output: Contribution to journalArticle

Feng, J, Dong, X, Pinello, J, Zhang, J, Lu, C, Iacob, RE, Engen, JR, Snell, WJ & Springer, TA 2018, 'Fusion surface structure, function, and dynamics of gamete fusogen HAP2', eLife, vol. 7. https://doi.org/10.7554/eLife.39772
Feng J, Dong X, Pinello J, Zhang J, Lu C, Iacob RE et al. Fusion surface structure, function, and dynamics of gamete fusogen HAP2. eLife. 2018 Oct 3;7. https://doi.org/10.7554/eLife.39772
Feng, Juan ; Dong, Xianchi ; Pinello, Jennifer ; Zhang, Jun ; Lu, Chafen ; Iacob, Roxana E. ; Engen, John R. ; Snell, William J. ; Springer, Timothy A. / Fusion surface structure, function, and dynamics of gamete fusogen HAP2. In: eLife. 2018 ; Vol. 7.
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