FV peptide induces apoptosis in HEp 2 and HeLa cells: An insight into the mechanism of induction

M. Sri Balasubashini, S. Karthigayan, S. T. Somasundaram, T. Balasubramanian, R. Rukkumani, Venugopal P. Menon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The present study is an attempt to evaluate the antiproliferative potential of peptide (7.6 kDa) from lionfish (Pterios volitans) venom on cultured HEp2 and HeLa cells. Different dose of purified peptide (1, 2 and 4 μg/ml) at different time points (12, 24 and 36 hrs) were tested for antiproliferative index of the peptide. Among them, 2 μg/ml at 24 hrs was found to effectively inhibit cancer cell growth in vitro and did not cause any adverse effect on normal human lymphocytes. Apoptosis was examined by propidium iodide staining, confirmed by the expression of caspase-8 and caspase-3, down regulation of Bcl-2 expression and DNA fragmentation in treated cells, when compared to untreated HEp2 and HeLa cells. Thus fish venom peptide was found to selectively induce apoptosis in cancer cell.

Original languageEnglish (US)
Article number27
JournalJournal of Carcinogenesis
Volume5
DOIs
StatePublished - Dec 1 2006

Fingerprint

HeLa Cells
Apoptosis
Peptides
Fish Venoms
Cells
Lymphocytes
Caspase 8
Propidium
Venoms
Cell growth
DNA Fragmentation
Caspase 3
Cultured Cells
Neoplasms
Down-Regulation
Staining and Labeling
DNA
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Cancer Research

Cite this

Sri Balasubashini, M., Karthigayan, S., Somasundaram, S. T., Balasubramanian, T., Rukkumani, R., & Menon, V. P. (2006). FV peptide induces apoptosis in HEp 2 and HeLa cells: An insight into the mechanism of induction. Journal of Carcinogenesis, 5, [27]. https://doi.org/10.1186/1477-3163-5-27

FV peptide induces apoptosis in HEp 2 and HeLa cells : An insight into the mechanism of induction. / Sri Balasubashini, M.; Karthigayan, S.; Somasundaram, S. T.; Balasubramanian, T.; Rukkumani, R.; Menon, Venugopal P.

In: Journal of Carcinogenesis, Vol. 5, 27, 01.12.2006.

Research output: Contribution to journalArticle

Sri Balasubashini, M, Karthigayan, S, Somasundaram, ST, Balasubramanian, T, Rukkumani, R & Menon, VP 2006, 'FV peptide induces apoptosis in HEp 2 and HeLa cells: An insight into the mechanism of induction', Journal of Carcinogenesis, vol. 5, 27. https://doi.org/10.1186/1477-3163-5-27
Sri Balasubashini M, Karthigayan S, Somasundaram ST, Balasubramanian T, Rukkumani R, Menon VP. FV peptide induces apoptosis in HEp 2 and HeLa cells: An insight into the mechanism of induction. Journal of Carcinogenesis. 2006 Dec 1;5. 27. https://doi.org/10.1186/1477-3163-5-27
Sri Balasubashini, M. ; Karthigayan, S. ; Somasundaram, S. T. ; Balasubramanian, T. ; Rukkumani, R. ; Menon, Venugopal P. / FV peptide induces apoptosis in HEp 2 and HeLa cells : An insight into the mechanism of induction. In: Journal of Carcinogenesis. 2006 ; Vol. 5.
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