Gallic acid is the major active component of cortex moutan in inhibiting immune maturation of human monocyte-derived dendritic cells

Ben Chung Lap Chan, Long Fei Li, Shui Qing Hu, Elaine Wat, Eric Chun Wai Wong, Vanilla Xin Zhang, Clara Bik San Lau, Chun Kwok Wong, Kam Lun Ellis Hon, Patrick Chi Leung Hui, Ping Chung Leung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Atopic dermatitis (AD) is a widely prevalent and chronically relapsing inflammatory skin disease. Penta Herbs Formula (PHF) is efficacious in improving the quality of life and reducing topical corticosteroid used in children with AD and one of the active herbs it contains is Cortex Moutan. Recent studies showed that altered functions of dendritic cells (DC) were observed in atopic individuals, suggesting that DC might play a major role in the generation and maintenance of inflammation by their production of pro-inflammatory cytokines. Hence, the aims of the present study were to identify the major active component(s) of Cortex Moutan, which might inhibit DC functions and to investigate their possible interactions with conventional corticosteroid on inhibiting the development of DC from monocytes. Monocyte-derived dendritic cells (moDC) culture model coupled with the high-speed counter-current chromatography (HSCCC), high pressure liquid chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LCMS) analyses were used. Gallic acid was the major active component from Cortex Moutan which could dose dependently inhibit interleukin (IL)-12 p40 and the functional cluster of differentiation (CD) surface markers CD40, CD80, CD83 and CD86 expression from cytokine cocktail-activated moDC. Gallic acid could also lower the concentration of hydrocortisone required to inhibit the activation of DC.

Original languageEnglish (US)
Pages (from-to)16388-16403
Number of pages16
JournalMolecules
Volume20
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

monocytes
Gallic Acid
cortexes
Dendritic Cells
Monocytes
dermatitis
acids
corticosteroids
cells
liquid chromatography
Atopic Dermatitis
interleukins
Adrenal Cortex Hormones
CD Antigens
chromatography
High pressure liquid chromatography
Cytokines
markers
maintenance
Rubiaceae

Keywords

  • Atopic dermatitis
  • Cortex Moutan
  • Dendritic cells
  • Gallic acid
  • High-speed counter-current chromatography
  • Penta Herb Formula

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Gallic acid is the major active component of cortex moutan in inhibiting immune maturation of human monocyte-derived dendritic cells. / Chan, Ben Chung Lap; Li, Long Fei; Hu, Shui Qing; Wat, Elaine; Wong, Eric Chun Wai; Zhang, Vanilla Xin; Lau, Clara Bik San; Wong, Chun Kwok; Hon, Kam Lun Ellis; Hui, Patrick Chi Leung; Leung, Ping Chung.

In: Molecules, Vol. 20, No. 9, 01.09.2015, p. 16388-16403.

Research output: Contribution to journalArticle

Chan, BCL, Li, LF, Hu, SQ, Wat, E, Wong, ECW, Zhang, VX, Lau, CBS, Wong, CK, Hon, KLE, Hui, PCL & Leung, PC 2015, 'Gallic acid is the major active component of cortex moutan in inhibiting immune maturation of human monocyte-derived dendritic cells', Molecules, vol. 20, no. 9, pp. 16388-16403. https://doi.org/10.3390/molecules200916388
Chan, Ben Chung Lap ; Li, Long Fei ; Hu, Shui Qing ; Wat, Elaine ; Wong, Eric Chun Wai ; Zhang, Vanilla Xin ; Lau, Clara Bik San ; Wong, Chun Kwok ; Hon, Kam Lun Ellis ; Hui, Patrick Chi Leung ; Leung, Ping Chung. / Gallic acid is the major active component of cortex moutan in inhibiting immune maturation of human monocyte-derived dendritic cells. In: Molecules. 2015 ; Vol. 20, No. 9. pp. 16388-16403.
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