Gap junction communication modulates [Ca2+](i) oscillations and enzyme secretion in pancreatic acini

Peggy Loessberg Stauffer, Hong Zhao, Katherine Luby-Phelps, Robert L. Moss, Robert A. Star, Shmuel Muallem

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

Global (all cells in an acinus) and focal (1-2 out of 10-15 cells) stimulation of pancreatic acini with bombesin or t-butyloxycarbonyl- Tyr(SO3)-Nle-Gly-Tyr-Asp-2-phenylethyl ester (CCKJ) together with modulation of gap junction (GJ) permeability by octanol and NO2/- was used to study the role of GJ permeability in controlling [Ca2+](i) oscillations and enzyme secretion. GJ permeability was quantitated by measuring fluorescence recovery after photobleaching. Octanol at 0.5 mM markedly reduced, whereas 15 mM NO2/- increased GJ permeability. Focal application of bombesin caused synchronized oscillations in the entire acinus, whereas global stimulation resulted in asynchronous oscillations. Increasing GJ permeability with NO2/- had no effect on bombesin-evoked [Ca2+](i) oscillations. Octanol inhibited ongoing oscillations evoked by focal or global bombesin stimulation. However, when GJ were blocked prior to stimulation, subsequent global stimulation with bombesin induced long-lasting oscillations in all cells. Re-establishing GJ communication for as little as 37.5 s conferred GJ dependence on the order and time of [Ca2+](i) spiking evoked by global bombesin stimulation. Focal and global stimulation with CCKJ gave different patterns of [Ca2+](i) oscillations. However, in contrast to bombesin, inhibition of GJ with octanol had no effect on oscillations induced by global CCKJ stimulation. Increasing GJ permeability with NO2/- synchronized CCKJ- stimulated oscillations by equalizing the amplitude and increasing the frequency in all cells within an acinus. These observations suggest that amplitude and frequency of [Ca2+](i) oscillations can be regulated independently of each other, and that GJ permeable molecules modulate the frequency of [Ca2+](i) oscillation in an agonist-specific manner. Regardless of the agonist, increasing the frequency of oscillations by modulation of GJ permeability correlated with an increased enzyme secretion.

Original languageEnglish (US)
Pages (from-to)19769-19775
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number26
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Stauffer, P. L., Zhao, H., Luby-Phelps, K., Moss, R. L., Star, R. A., & Muallem, S. (1993). Gap junction communication modulates [Ca2+](i) oscillations and enzyme secretion in pancreatic acini. Journal of Biological Chemistry, 268(26), 19769-19775.