GCNA Preserves Genome Integrity and Fertility Across Species

Varsha Bhargava, Courtney D. Goldstein, Logan Russell, Lin Xu, Murtaza Ahmed, Wei Li, Amanda Casey, Kelly Servage, Rahul Kollipara, Zachary Picciarelli, Ralf Kittler, Alexander Yatsenko, Michelle Carmell, Kim Orth, James F Amatruda, Judith L. Yanowitz, Michael Buszczak

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


The propagation of species depends on the ability of germ cells to protect their genome from numerous exogenous and endogenous threats. While these cells employ ubiquitous repair pathways, specialized mechanisms that ensure high-fidelity replication, chromosome segregation, and repair of germ cell genomes remain incompletely understood. We identified Germ Cell Nuclear Acidic Peptidase (GCNA) as a conserved regulator of genome stability in flies, worms, zebrafish, and human germ cell tumors. GCNA contains an acidic intrinsically disordered region (IDR) and a protease-like SprT domain. In addition to chromosomal instability and replication stress, Gcna mutants accumulate DNA-protein crosslinks (DPCs). GCNA acts in parallel with the SprT domain protein Spartan. Structural analysis reveals that while the SprT domain is needed to limit DNA damage, the IDR imparts significant function. This work shows that GCNA protects germ cells from various sources of damage, providing insights into conserved mechanisms that promote genome integrity across generations.

Original languageEnglish (US)
Pages (from-to)38-52.e10
JournalDevelopmental cell
Issue number1
StatePublished - Jan 6 2020


  • ACRC
  • DNA repair
  • DNA-protein crosslink
  • SprT domain
  • acidic repeat containing
  • chromosome instability
  • chromosome segregation
  • germ cell
  • intrinsically disordered region
  • transgenerational inheritance

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology


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