GEC1, a protein related to GABARAP, interacts with tubulin and GABA A receptor

Virginie Mansuy, Wilfrid Boireau, Annick Fraichard, Jean Luc Schlick, Michèle Jouvenot, Régis Delage-Mourroux

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

We have previously identified in uterine cells a novel estrogen-regulated gene called gec1. GEC1 presents 87% identity with GABARAP which, so far, was the only protein found to associate with tubulin and GABA A receptor. We demonstrated then that GEC1 interacts in vitro with tubulin and GABA A receptor, and promotes tubulin assembly and microtubule bundling. Since all polyclonal antibodies failed in discrimination of both proteins GEC1 and GABARAP, a GEC1-GFP fusion protein was used to specifically localize GEC1. GEC1-GFP was distributed over the cytoplasm in perinuclear vesicles with a scattered pattern. Overall, our data show that GEC1 could be a new member of the GABARAP family involved in the transport of GABA A receptor.

Original languageEnglish (US)
Pages (from-to)639-648
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume325
Issue number2
DOIs
StatePublished - Dec 10 2004

Keywords

  • Biacore
  • GABARAP
  • GEC1
  • GST-pull-down
  • Protein trafficking
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'GEC1, a protein related to GABARAP, interacts with tubulin and GABA <sub>A</sub> receptor'. Together they form a unique fingerprint.

Cite this