Gene expression monitoring for gene discovery in models of peripheral and central nervous system differentiation, regeneration, and trauma

Deborah Norman Farlow, Gordon Vansant, Adrian A. Cameron, Jenny Chang, Su Khoh-Reiter, Nhat Long Pham, Wen Wu, Yutaka Sagara, John G. Nicholls, Dennis J. Carlo, R. Charles

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Gene expression monitoring using gene expression microarrays represents an extremely powerful technology for gene discovery in a variety of systems. We describe the results of seven experiments using Incyte GEM technology to compile a proprietary portfolio of data concerning differential gene expression in six different models of neuronal differentiation and regeneration, and recovery from injury or disease. Our first two experiments cataloged genes significantly up- or down-regulated during two phases of the retinoic acid-induced differentiation of the embryonal carcinoma line Ntera-2. To identify genes involved in neuronal regeneration we performed three GEM experiments, which included changes in gene expression in rat dorsal root ganglia during the healing of experimentally injured sciatic nerve, in regenerating neonatal opossum spinal cord, and during lipopolysaccharide stimulation of primary cultures of rat Schwann cells. Finally we have monitored genes involved in the recovery phase of the inflammatory disease of the rat spinal cord, experimental allergic encephalomyelitis, as well as those responsible for protection from oxidative stress in a glutamate-resistant rat hippocampal cell line. Analysis of the results of the approximately 70,000 data points collected is presented.

Original languageEnglish (US)
Pages (from-to)171-180
Number of pages10
JournalJournal of Cellular Biochemistry
Volume80
Issue number2
DOIs
StatePublished - Feb 1 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Farlow, D. N., Vansant, G., Cameron, A. A., Chang, J., Khoh-Reiter, S., Pham, N. L., Wu, W., Sagara, Y., Nicholls, J. G., Carlo, D. J., & Charles, R. (2001). Gene expression monitoring for gene discovery in models of peripheral and central nervous system differentiation, regeneration, and trauma. Journal of Cellular Biochemistry, 80(2), 171-180. https://doi.org/10.1002/1097-4644(20010201)80:2<171::AID-JCB20>3.0.CO;2-5