Gene expressions associated with chemosensitivity in human hepatoma cells

Yujin Hoshida, Masaru Moriyama, Motoyuki Otsuka, Naoya Kato, Hiroyoshi Taniguchi, Yasushi Shiratori, Naohiko Seki, Masao Omata

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Background/Aims: Only limited patients with hepatoma benefit from chemotherapy without a clear explanation. We aimed to identify genes associated with chemosensitivity using transcriptional profiles. Methodology: In 8 hepatoma cells (HLE, HLF, Huh7, Hep3B, PLC/PRF/5, SK-Hep1, Huh6, and HepG2) transcriptional profiles were obtained using cDNA microarray including 2,300 genes. Chemosensitivities to 8 anticancer drugs (nimustine, mitomycin C, cisplatin, carboplatin, doxorubicin, epirubicin, mitoxantrone, and 5-fluorouracil) were measured by obtaining 50% growth inhibitory concentrations (GI50) using MTT assay. Genes having drug-specific association with chemosensitivity were selected. Results: Up-regulation of topoisomerase II beta was associated with chemo-resistance, the target of doxorubicin. Platinum-specific resistance was associated with superoxide dismutase 2 expression. Antigen peptide transporter 1 expression correlated with nimustine and mitoxantrone-specific susceptibility. These results were verified by semi-quantitative RT-PCR. Drug inactivators reported in non-liver cancers such as multidrug transporters and drug metabolizers showed less diversity of chemosensitivity in hepatoma cells. Conclusions: To evaluate these gene expressions may be useful to select anticancer drugs, and possibly to consider new therapeutic target to modify drug action.

Original languageEnglish (US)
Pages (from-to)489-492
Number of pages4
JournalHepato-Gastroenterology
Volume54
Issue number74
Publication statusPublished - Mar 1 2007
Externally publishedYes

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Keywords

  • Anticancer drug
  • Chemosensitivity
  • Gene expression
  • Hepatoma

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Hoshida, Y., Moriyama, M., Otsuka, M., Kato, N., Taniguchi, H., Shiratori, Y., ... Omata, M. (2007). Gene expressions associated with chemosensitivity in human hepatoma cells. Hepato-Gastroenterology, 54(74), 489-492.