Gene targeted ablation of high molecular weight fibroblast growth factor-2

Mohamad Azhar, Moying Yin, Ming Zhou, Hongqi Li, Marwan Mustafa, Eyad Nusayr, Jack B. Keenan, Hwudaurw Chen, Sharon Pawlosky, Connie Gard, Christina Grisham, L. Philip Sanford, Tom Doetschman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Fibroblast growth factor-2 (FGF2) is produced as high molecular weight isoforms (HMW) and a low molecular weight isoform (LMW) by means of alternative usage of translation start sites in a single Fgf2 mRNA. Although the physiological function of FGF2 and FGF2 LMW has been investigated in myocardial capillarogenesis during normal cardiac growth, the role of FGF2 HMW has not been determined. Here, we report the generation of FGF2 HMW-deficient mice in which FGF2 HMW isoforms are ablated by the Tag-and-Exchange gene targeting technique. These mice are normal and fertile with normal fecundity, and have a normal life span. Histological, immunohistochemical, and morphometric analyses indicate normal myocardial architecture, blood vessel, and cardiac capillary density in young adult FGF2 HMW-deficient mice. These mice along with the FGF2- and FGF2 LMW-deficient mice that we have generated previously will be very useful for elucidating the differential functions of FGF2 isoforms in pathophysiology of cardiovascular diseases.

Original languageEnglish (US)
Pages (from-to)351-357
Number of pages7
JournalDevelopmental Dynamics
Volume238
Issue number2
DOIs
StatePublished - Feb 1 2009

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Keywords

  • Angiogenesis
  • Bone formation
  • Cardiac capillary
  • Cardiac hypertrophy
  • Fibroblast growth factor
  • High molecular weight isoforms
  • Ischemia reperfusion-injury
  • Knockout mice
  • Neurogenesis
  • Single nucleotide polymorphism
  • Tag and exchange
  • Tumor

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Azhar, M., Yin, M., Zhou, M., Li, H., Mustafa, M., Nusayr, E., Keenan, J. B., Chen, H., Pawlosky, S., Gard, C., Grisham, C., Sanford, L. P., & Doetschman, T. (2009). Gene targeted ablation of high molecular weight fibroblast growth factor-2. Developmental Dynamics, 238(2), 351-357. https://doi.org/10.1002/dvdy.21835