Generation of superoxide anion by brain endothelial cell xanthine oxidase

Lance S. Terada, Irene R. Willingham, Mary E. Rosandich, Jonathan A. Leff, Glenn W. Kindt, John E. Repine

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Abstract

Bovine brain endothelial cells (EC) that were isolated and propagated in pure culture had increased ( 20‐fold) levels of xanthine oxidase and xanthine dehydrogenase activity compared to whole brain homogenate. Brain EC also released superoxide anion (O 2−) in to the extracellular medium. Treatment of EC with tungsten decreased (P < 0.05), both XO activity and O 2− release. XO appears to be highly concentrated in cerebral vascular endothelium and may be an important source o; O 2−.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalJournal of Cellular Physiology
Volume148
Issue number2
DOIs
StatePublished - Aug 1991

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ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Terada, L. S., Willingham, I. R., Rosandich, M. E., Leff, J. A., Kindt, G. W., & Repine, J. E. (1991). Generation of superoxide anion by brain endothelial cell xanthine oxidase. Journal of Cellular Physiology, 148(2), 191-196. https://doi.org/10.1002/jcp.1041480202