Genetic analysis of intestinal Cholesterol absorption in inbred mice

M. Schwarz, D. L. Davis, B. R. Vick, D. W. Russell

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

A genetic mapping strategy was employed to identify chromosomal regions harboring genes that influence the absorption of intestinal cholesterol in the mouse. Analysis of seven inbred strains of male mice (129P3, AKR, BALB/c, C3H/He, C57BL/6, DBA/2, and SJL, all from Jackson Laboratories) revealed substantial differences in their abilities to absorb a bolus of cholesterol delivered by gavage. Crosses between high (AKR, 129) and low (DBA/2, SJL) absorbing strains revealed evidence for the presence of dominant genes that increase and decrease cholesterol absorption. Backcrosses between F1 offspring and parental strains (DBA/2xAKD2F1 and 129xSJL129F1) followed by linkage analyses revealed four quantitative trait loci that influenced cholesterol absorption. Analyses of recombinant inbred strains identified an additional three loci affecting this phenotype. These seven quantitative trait loci, which map to different chromosomes and are termed Cholesterol absorption 1-7 (Chab1-7) loci, together influence the absorption of intestinal cholesterol in mice and are likely to be involved in different steps of this complex pathway.

Original languageEnglish (US)
Pages (from-to)1801-1811
Number of pages11
JournalJournal of lipid research
Volume42
Issue number11
StatePublished - 2001

Keywords

  • Bile acids
  • Lipid metabolism
  • Quantitative trait mapping

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Fingerprint Dive into the research topics of 'Genetic analysis of intestinal Cholesterol absorption in inbred mice'. Together they form a unique fingerprint.

Cite this