Genetic and epigenetic inactivation of LTF gene at 3p21.3 in lung cancers

Hironobu Iijima, Yoshio Tomizawa, Yasuki Iwasaki, Koji Sato, Noriaki Sunaga, Kunio Dobashi, Ryusei Saito, Takashi Nakajima, John D. Minna, Masatomo Mori

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Allelic loss on the short arm of chromosome 3 is one of the most common events in the pathogenesis of lung cancer. The lactotransferrin gene (LTF, also referred to as the lactoferrin gene, LF) is located at 3p21.3 common eliminated region 1, which is frequently deleted in lung and other cancers. The expression of the LTF gene was absent in 16 (59%) of 27 small cell lung cancer cell lines, 33 (77%) of 43 nonsmall-cell lung cancer (NSCLC) cell lines and 7 (54%) of 13 primary NSCLC, while LTF mRNA was overexpressed in 3 (7%) of 43 NSCLC cell lines. Its expression was restored by treatment with 5-aza-2′- deoxycytidine (5-aza-dC), trichostatin A (TSA) or a combination of both in a subset of lung cancer cell lines without LTF expression. In addition, we found 8 different types of nucleotide substitutions and one frameshift mutation. These results indicate that the LTF gene is inactivated by genetic and epigenetic mechanisms in lung cancer.

Original languageEnglish (US)
Pages (from-to)797-801
Number of pages5
JournalInternational Journal of Cancer
Volume118
Issue number4
DOIs
StatePublished - Feb 15 2006

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Keywords

  • 3p21.3
  • Deacetylation
  • Lactotransferrin
  • Lung cancer
  • Methylation
  • Mutation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Iijima, H., Tomizawa, Y., Iwasaki, Y., Sato, K., Sunaga, N., Dobashi, K., Saito, R., Nakajima, T., Minna, J. D., & Mori, M. (2006). Genetic and epigenetic inactivation of LTF gene at 3p21.3 in lung cancers. International Journal of Cancer, 118(4), 797-801. https://doi.org/10.1002/ijc.21462