Genetic dissection of the murine lupus susceptibility locus Sle2: Contributions to increased peritoneal B-1a cells and lupus nephritis map to different loci

Zhiwei Xu, Biyan Duan, Byron P. Croker, Edward K. Wakeland, Laurence Morel

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Lupus pathogenesis in the NZM2410 mouse model results from the expression of multiple interacting susceptibility loci. Sle2 on chromosome 4 was significantly linked to glomerulonephritis in a linkage analysis of a NZM2410 × B6 cross. Yet, Sle2 expression alone on a C57BL/6 background did not result in any clinical manifestation, but in an abnormal B cell development, including the accumulation of B-1a cells in the peritoneal cavity and spleen. Analysis of B6.Sle2 congenic recombinants showed that at least three independent loci, New Zealand White-derived Sle2a and Sle2b, and New Zealand Black-derived Sle2c, contribute to an elevated number of B-1a cells, with Sle2c contribution being the strongest of the three. To determine the contribution of these three Sle2 loci to lupus pathogenesis, we used a mapping by genetic interaction strategy, in which we bred them to B6.Sle1.Sle3 mice. We then compared the phenotypes of these triple congenic mice with that of previously characterized B6.Sle1.Sle2.Sle3, which express the entire Sle2 interval in combination with Sle1 and Sle3. Sle2a and Sle2b, but not Sle2c, contributed significantly to lupus pathogenesis in terms of survival rate, lymphocytic expansion, and kidney pathology. These results show that the Sle2 locus contains several loci affecting B cell development, with only the two NZW-derived loci having the least effect of B-1a cell accumulation significantly contributing to lupus pathogenesis.

Original languageEnglish (US)
Pages (from-to)936-943
Number of pages8
JournalJournal of Immunology
Volume175
Issue number2
DOIs
StatePublished - Jul 15 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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