Genetic inactivation of NPC1L1 protects against sitosterolemia in mice lacking ABCG5/ABCG8

Weiqing Tang, Yinyan Ma, Lin Jia, Yiannis A. Ioannou, Joanna P. Davies, Liqing Yu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Mice lacking Niemann-Pick C1-Like 1 (NPC1L1) (NPC1L1-/-mice) exhibit a defect in intestinal absorption of cholesterol and phytosterols. However, wild-type (WT) mice do not efficiently absorb and accumulate phytosterols either. Cell-based studies show that NPC1L1 is a much weaker transporter for phytosterols than cholesterol. In this study, we examined the role of NPC1L1 in phytosterol and cholesterol trafficking in mice lacking ATP-binding cassette (ABC) transporters G5 and G8 (G5/G8-/-mice). G5/G8-/-mice develop sitosterolemia, a genetic disorder characterized by the accumulation of phytosterols in blood and tissues. We found that mice lacking ABCG5/G8 and NPC1L1 [triple knockout (TKO) mice] did not accumulate phytosterols in plasma and the liver. TKO mice, like G5/G8-/-mice, still had a defect in hepatobiliary cholesterol secretion, which was consistent with TKO versus NPC1L1-/- mice exhibiting a 52% reduction in fecal cholesterol excretion. Because fractional cholesterol absorption was reduced similarly in NPC1L1-/- and TKO mice, by subtracting fecal cholesterol excretion in TKO mice from NPC1L1-/-mice, we estimated that a 25g NPC1L1-/-mouse may secrete about 4 μmol of cholesterol daily via the G5/G8 pathway. In conclusion, NPC1L1 is essential for phytosterols to enter the body in mice.

Original languageEnglish (US)
Pages (from-to)293-300
Number of pages8
JournalJournal of Lipid Research
Volume50
Issue number2
DOIs
StatePublished - Jul 9 2009

Fingerprint

Phytosterols
Cholesterol
Knockout Mice
Defects
ATP-Binding Cassette Transporters
Sitosterolemia
Liver
Inborn Genetic Diseases
Blood
Intestinal Absorption
Tissue
Plasmas

Keywords

  • ATP-binding cassette
  • Niemann-Pick C1-Like 1
  • Phytosterols
  • Sterol absorption

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Genetic inactivation of NPC1L1 protects against sitosterolemia in mice lacking ABCG5/ABCG8. / Tang, Weiqing; Ma, Yinyan; Jia, Lin; Ioannou, Yiannis A.; Davies, Joanna P.; Yu, Liqing.

In: Journal of Lipid Research, Vol. 50, No. 2, 09.07.2009, p. 293-300.

Research output: Contribution to journalArticle

Tang, Weiqing ; Ma, Yinyan ; Jia, Lin ; Ioannou, Yiannis A. ; Davies, Joanna P. ; Yu, Liqing. / Genetic inactivation of NPC1L1 protects against sitosterolemia in mice lacking ABCG5/ABCG8. In: Journal of Lipid Research. 2009 ; Vol. 50, No. 2. pp. 293-300.
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