Genetic mouse models: The powerful tools to study fat tissues

Xingxing Kong, Kevin W. Williams, Tiemin Liu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Obesity and Type 2 diabetes (T2D) are associated with a variety of comorbidities that contribute to mortality around the world. Although significant effort has been expended in understanding mechanisms that mitigate the consequences of this epidemic, the field has experienced limited success thus far. The potential ability of brown adipose tissue (BAT) to counteract obesity and metabolic disease in rodents (and potentially in humans) has been a topical realization. Recently, there is also another thermogenic fat cell called beige adipocytes, which are located among white adipocytes and share similar activated responses to cyclic AMP as classical BAT. In this chapter, we review contemporary molecular strategies to investigate the role of adipose tissue depots in metabolism. In particular, we will discuss the generation of adipose tissue-specific knockout and overexpression of target genes in various mouse models. We will also discuss how to use different Cre (cyclization recombination) mouse lines to investigate diverse types of adipocytes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages99-107
Number of pages9
Volume1566
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1566
ISSN (Print)10643745

Fingerprint

Brown Adipose Tissue
Genetic Models
Adipocytes
Adipose Tissue
Obesity
White Adipocytes
Fats
Metabolic Diseases
Cyclization
Cyclic AMP
Type 2 Diabetes Mellitus
Genetic Recombination
Comorbidity
Rodentia
Mortality
Genes
Beige Adipocytes

Keywords

  • Adiponectin-cre
  • Beige adipocyte
  • Rosa26-loxp-stop-loxp
  • Ucp1-cre

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kong, X., Williams, K. W., & Liu, T. (2017). Genetic mouse models: The powerful tools to study fat tissues. In Methods in Molecular Biology (Vol. 1566, pp. 99-107). (Methods in Molecular Biology; Vol. 1566). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6820-6_10

Genetic mouse models : The powerful tools to study fat tissues. / Kong, Xingxing; Williams, Kevin W.; Liu, Tiemin.

Methods in Molecular Biology. Vol. 1566 Humana Press Inc., 2017. p. 99-107 (Methods in Molecular Biology; Vol. 1566).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kong, X, Williams, KW & Liu, T 2017, Genetic mouse models: The powerful tools to study fat tissues. in Methods in Molecular Biology. vol. 1566, Methods in Molecular Biology, vol. 1566, Humana Press Inc., pp. 99-107. https://doi.org/10.1007/978-1-4939-6820-6_10
Kong X, Williams KW, Liu T. Genetic mouse models: The powerful tools to study fat tissues. In Methods in Molecular Biology. Vol. 1566. Humana Press Inc. 2017. p. 99-107. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6820-6_10
Kong, Xingxing ; Williams, Kevin W. ; Liu, Tiemin. / Genetic mouse models : The powerful tools to study fat tissues. Methods in Molecular Biology. Vol. 1566 Humana Press Inc., 2017. pp. 99-107 (Methods in Molecular Biology).
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