Glucagon-like peptide-1-responsive catecholamine neurons in the area postrema link peripheral glucagon-like peptide-1 with central autonomic control sites

Hiroshi Yamamoto, Toshiro Kishi, Charlotte E. Lee, Brian J. Choi, Hui Fang, Anthony N. Hollenberg, Daniel J. Drucker, Joel K. Elmquist

Research output: Contribution to journalArticlepeer-review

199 Scopus citations

Abstract

Glucagon-like peptide-1 (GLP-1) released from the gut is an incretin that stimulates insulin secretion. GLP-1 is also a brain neuropeptide that has diverse central actions, including inhibition of food and water intake, gastric emptying, and stimulation of neuroendocrine responses characteristic of visceral illness. Both intravenous and intracerebroventricular administration of GLP-1 receptor (GLP-1R) agonists increase blood pressure and heart rate and induce Fos-like immunoreactivity (Fos-IR) in autonomic regulatory sites in the rat brain. The area postrema (AP) is a circumventricular organ and has been implicated in processing visceral sensory information. GLP-1Rs are densely expressed in the AP, and peripheral GLP-1R agonists induce Fos-IR in AP neurons to a greater degree than intracerebroventricular administration. Because the AP lacks a blood-brain barrier, we hypothesized that the AP is a key site for peripheral GLP-1 to activate central autonomic regulatory sites. In this study, we found that many tyrosine hydroxylase (TH)-containing neurons in the AP expressed GLP-1Rs and Fos-IR after intravenous GLP-1R agonists. Furthermore, intravenous but not intracerebroventricular GLP-1R agonists induced TH transcription in the AP in vivo. In addition, GLP-1R agonists directly activated TH transcription in an in vitro cell system. Finally, we found that GLP-1-responsive TH neurons in the AP innervate autonomic control sites, including the parabrachial nucleus, nucleus of solitary tract, and ventrolateral medulla. These findings suggest that catecholamine neurons in the AP link peripheral GLP-1 and central autonomic control sites that mediate the diverse neuroendocrine and autonomic actions of peripheral GLP-1.

Original languageEnglish (US)
Pages (from-to)2939-2946
Number of pages8
JournalJournal of Neuroscience
Volume23
Issue number7
DOIs
StatePublished - Apr 1 2003

Keywords

  • Area postrema
  • Autonomic regulatory system
  • Blood-brain barrier
  • Catecholamine neurons
  • GLP-1
  • Insulin
  • Tyrosine hydroxylase
  • c-Fos

ASJC Scopus subject areas

  • Neuroscience(all)

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