Gram-negative bacterial lipopolysaccharide promotes tumor cell proliferation in breast implant-associated anaplastic large-cell lymphoma

Maria Mempin, Honghua Hu, Karen Vickery, Marshall E. Kadin, H. Miles Prince, Nicola Kouttab, John W. Morgan, William P. Adams, Anand K. Deva

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Breast implant-associated anaplastic large-cell lymphoma (BIA-ALCL) is a distinct malignancy associated with textured breast implants. We investigated whether bacteria could trigger the activation and multiplication of BIA-ALCL cells in vitro. BIA-ALCL patient-derived BIA-ALCL tumor cells, BIA-ALCL cell lines, cutaneous ALCL cell lines, an immortal T-cell line (MT-4), and peripheral blood mononuclear cells (PBMC) from BIA-ALCL, capsular contracture, and primary augmentation patients were studied. Cells were subjected to various mitogenic stimulation assays including plant phytohemagglutinin (PHA), Gram-negative bacterial lipopolysaccharide (LPS), Staphylococcal superantigens enterotoxin A (SEA), toxic shock syndrome toxin-1 (TSST-1), or steril-ized implant shells. Patient-derived BIA-ALCL tumor cells and BIA-ALCL cell lines showed a unique response to LPS stimulation. This response was dampened significantly in the presence of a Toll-like receptor 4 (TLR4) inhibitor peptide. In contrast, cutaneous ALCL cells, MT-4, and PBMC cells from all patients responded significantly more to PHA, SEA, and TSST-1 than to LPS. Breast implant shells of all surface grades alone did not produce a proliferative response of BIA-ALCL cells, indicating the breast implant does not act as a pro-inflammatory stimulant. These findings indicate a possible novel pathway for LPS to promote BIA-ALCL cell proliferation via a TLR4 receptor-mediated bacterial transformation of T-cells into malignancy.

Original languageEnglish (US)
Article number5298
JournalCancers
Volume13
Issue number21
DOIs
StatePublished - Nov 1 2021

Keywords

  • Breast implant-associated anaplastic large-cell lymphoma
  • Lipopolysaccharide
  • Proliferation
  • T-cells’ malignancy
  • Tumor cell

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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