GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg]ZO-1) domain

Bryan E. Snow, Randy A. Hall, Andrejs M. Krumins, Greg M. Brothers, Denis Bouchard, Carol Anne Brothers, Stephen Chung, Joan Mangion, Alfred G. Gilman, Robert J. Lefkowitz, David P. Siderovski

Research output: Contribution to journalArticle

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Abstract

Regulator of G-protein signaling (RGS) proteins increase the intrinsic guanosine triphosphatase (GTPase) activity of G-protein α subunits in vitro, but how specific G-protein-coupled receptor systems are targeted for down- regulation by RGS proteins remains uncharacterized. Here, we describe the GTPase specificity of RGS12 and identify four alternatively spliced forms of human RGS12 mRNA. Two RGS12 isoforms of 6.3 and 5.7 kilobases (kb), encoding both an N-terminal PDZ (PSD-95/DIg/ZO-1) domain and the RGS domain, are expressed in most tissues, with highest levels observed in testis, ovary, spleen, cerebellum, and caudate nucleus. The 5.7-kb isoform has an alternative 3' end encoding a putative C-terminal PDZ domain docking site. Two smaller isoforms, of 3.1 and 3.7 kb, which lack the PDZ domain and encode the RGS domain with and without the alternative 3' end, respectively, are most abundantly expressed in brain, kidney, thymus, and prostate. In vitro biochemical assays indicate that RGS12 is a GTPase-activating protein for G(i) class α subunits. Biochemical and interaction trap experiments suggest that the RGS12 N terminus acts as a classical PDZ domain, binding selectively to C-terminal (A/S)-T-X-(L/V) motifs as found within both the interleukin-8 receptor B (CXCR2) and the alternative 3' exon form of RGS12. The presence of an alternatively spliced PDZ domain within RGS12 suggests a mechanism by which RGS proteins may target specific G-protein-coupled receptor systems for desensitization.

Original languageEnglish (US)
Pages (from-to)17749-17755
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number28
DOIs
StatePublished - Jul 10 1998

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RGS Proteins
Guanosine
GTP-Binding Proteins
GTP-Binding Protein Regulators
Protein Isoforms
G-Protein-Coupled Receptors
Interleukin-8B Receptors
Thymus
Caudate Nucleus
Protein Subunits
Cerebellum
Thymus Gland
Testis
Prostate
Ovary
Exons
Assays
Brain
Down-Regulation
Spleen

ASJC Scopus subject areas

  • Biochemistry

Cite this

Snow, B. E., Hall, R. A., Krumins, A. M., Brothers, G. M., Bouchard, D., Brothers, C. A., ... Siderovski, D. P. (1998). GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg]ZO-1) domain. Journal of Biological Chemistry, 273(28), 17749-17755. https://doi.org/10.1074/jbc.273.28.17749

GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg]ZO-1) domain. / Snow, Bryan E.; Hall, Randy A.; Krumins, Andrejs M.; Brothers, Greg M.; Bouchard, Denis; Brothers, Carol Anne; Chung, Stephen; Mangion, Joan; Gilman, Alfred G.; Lefkowitz, Robert J.; Siderovski, David P.

In: Journal of Biological Chemistry, Vol. 273, No. 28, 10.07.1998, p. 17749-17755.

Research output: Contribution to journalArticle

Snow, BE, Hall, RA, Krumins, AM, Brothers, GM, Bouchard, D, Brothers, CA, Chung, S, Mangion, J, Gilman, AG, Lefkowitz, RJ & Siderovski, DP 1998, 'GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg]ZO-1) domain', Journal of Biological Chemistry, vol. 273, no. 28, pp. 17749-17755. https://doi.org/10.1074/jbc.273.28.17749
Snow, Bryan E. ; Hall, Randy A. ; Krumins, Andrejs M. ; Brothers, Greg M. ; Bouchard, Denis ; Brothers, Carol Anne ; Chung, Stephen ; Mangion, Joan ; Gilman, Alfred G. ; Lefkowitz, Robert J. ; Siderovski, David P. / GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg]ZO-1) domain. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 28. pp. 17749-17755.
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AU - Bouchard, Denis

AU - Brothers, Carol Anne

AU - Chung, Stephen

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