Gut microbiota contributes to spontaneous colitis in e3 ligase itch-deficient mice

Mahesh Kathania, Elviche L. Tsakem, Arianne L. Theiss, K. Venuprasad

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Inflammatory bowel diseases are associated with complex shifts in microbiota composition. However, it remains unclear whether specific subsets of commensal bacteria induce inflammatory bowel diseases in genetically susceptible hosts. In this study, we found that deficiency of the E3 ligase Itch, which leads to spontaneous colitis and rectal prolapse, is associated with alteration of the gut microbiota. 16S rRNA sequencing showed expansion of colitogenic Bacteroides sp. in Itch-/- mice. Treatment with broad-spectrum antibiotics substantially reduced colonic inflammation in Itch-/- mice. Microbiota of Itch-/- mice failed to induce spontaneous colitis upon transfer to Itch+/+ mice but aggravated chemically induced colitis. Furthermore, we found that Bacteroides vulgatus, which is expanded in Itch-/- mice, was sufficient to induce colon inflammation in Itch-/- mice.

Original languageEnglish (US)
Pages (from-to)2277-2284
Number of pages8
JournalJournal of Immunology
Issue number8
StatePublished - Apr 15 2020

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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