TY - JOUR
T1 - Gut microbiota contributes to spontaneous colitis in e3 ligase itch-deficient mice
AU - Kathania, Mahesh
AU - Tsakem, Elviche L.
AU - Theiss, Arianne L.
AU - Venuprasad, K.
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health (R01-DK115668-01), the Cancer Prevention Research Institute of Texas (RP160577), Baylor Charles A. Sammons Cancer Center and Baylor Scott and White Research Institute–Translational Genomics Research Institute collaborative grants (to K.V.), and Grant R01-DK117001 from the National Institutes of Health (to A.L.T.).
Publisher Copyright:
© 2020 by The American Association of Immunologists, Inc.
PY - 2020/4/15
Y1 - 2020/4/15
N2 - Inflammatory bowel diseases are associated with complex shifts in microbiota composition. However, it remains unclear whether specific subsets of commensal bacteria induce inflammatory bowel diseases in genetically susceptible hosts. In this study, we found that deficiency of the E3 ligase Itch, which leads to spontaneous colitis and rectal prolapse, is associated with alteration of the gut microbiota. 16S rRNA sequencing showed expansion of colitogenic Bacteroides sp. in Itch-/- mice. Treatment with broad-spectrum antibiotics substantially reduced colonic inflammation in Itch-/- mice. Microbiota of Itch-/- mice failed to induce spontaneous colitis upon transfer to Itch+/+ mice but aggravated chemically induced colitis. Furthermore, we found that Bacteroides vulgatus, which is expanded in Itch-/- mice, was sufficient to induce colon inflammation in Itch-/- mice.
AB - Inflammatory bowel diseases are associated with complex shifts in microbiota composition. However, it remains unclear whether specific subsets of commensal bacteria induce inflammatory bowel diseases in genetically susceptible hosts. In this study, we found that deficiency of the E3 ligase Itch, which leads to spontaneous colitis and rectal prolapse, is associated with alteration of the gut microbiota. 16S rRNA sequencing showed expansion of colitogenic Bacteroides sp. in Itch-/- mice. Treatment with broad-spectrum antibiotics substantially reduced colonic inflammation in Itch-/- mice. Microbiota of Itch-/- mice failed to induce spontaneous colitis upon transfer to Itch+/+ mice but aggravated chemically induced colitis. Furthermore, we found that Bacteroides vulgatus, which is expanded in Itch-/- mice, was sufficient to induce colon inflammation in Itch-/- mice.
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U2 - 10.4049/jimmunol.1701478
DO - 10.4049/jimmunol.1701478
M3 - Article
C2 - 32169841
AN - SCOPUS:85084091885
VL - 204
SP - 2277
EP - 2284
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 8
ER -