H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes

Eric G. Pamer, Chyung Ru Wang, Lorraine Flaherty, Kirsten Fischer Lindahl, Michael J. Bevan

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

We report evidence that a major histocompatibility complex-encoded nonclassic class I molecule presents a foreign peptide to cytotoxic T lymphocytes (CTL) during an infection. Mice immunized with virulent Listeria monocytogenes generate CD8+ CTL with αβ receptors specific for a bacterial peptide presented by a conserved class I molecule encoded in the M region of the major histocompatibility complex. The Listeria peptide is digested by carboxypeptidase Y but resists aminopeptidase M, and only peptides with N-formyl methionine competitively block its presentation to CTL. Transfection with the H-2M3d gene enables a negative (H-2w17) cell line to present the bacterial peptide. One function, therefore, of H-2M3 is to present bacterial peptides to CTL during infection.

Original languageEnglish (US)
Pages (from-to)215-223
Number of pages9
JournalCell
Volume70
Issue number2
DOIs
StatePublished - Jul 24 1992

Fingerprint

Listeria
T-cells
Listeria monocytogenes
Cytotoxic T-Lymphocytes
Peptides
Major Histocompatibility Complex
Cathepsin A
CD13 Antigens
Molecules
Infection
Methionine
Transfection
Genes
Cells
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Pamer, E. G., Wang, C. R., Flaherty, L., Lindahl, K. F., & Bevan, M. J. (1992). H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes. Cell, 70(2), 215-223. https://doi.org/10.1016/0092-8674(92)90097-V

H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes. / Pamer, Eric G.; Wang, Chyung Ru; Flaherty, Lorraine; Lindahl, Kirsten Fischer; Bevan, Michael J.

In: Cell, Vol. 70, No. 2, 24.07.1992, p. 215-223.

Research output: Contribution to journalArticle

Pamer, EG, Wang, CR, Flaherty, L, Lindahl, KF & Bevan, MJ 1992, 'H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes', Cell, vol. 70, no. 2, pp. 215-223. https://doi.org/10.1016/0092-8674(92)90097-V
Pamer EG, Wang CR, Flaherty L, Lindahl KF, Bevan MJ. H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes. Cell. 1992 Jul 24;70(2):215-223. https://doi.org/10.1016/0092-8674(92)90097-V
Pamer, Eric G. ; Wang, Chyung Ru ; Flaherty, Lorraine ; Lindahl, Kirsten Fischer ; Bevan, Michael J. / H-2M3 presents a listeria monocytogenes peptide to cytotoxic T lymphocytes. In: Cell. 1992 ; Vol. 70, No. 2. pp. 215-223.
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