HBV C promoter Sp1 binding sequence functionally substitutes for the yeast ARS1 ABF1 binding site

Peijun Yan, Xicheng Mao, Lei Wang, Xiliang Zha, Changde Lu

Research output: Contribution to journalArticle

3 Scopus citations


Transcriptional factors have been implicated in eukaryotic DNA replication. We have studied the potential function of a viral promoter sequence in DNA replication. The hepatitis B virus (HBV) pregenomic promoter is regulated by two enhancers and cis-elements. The G-C rich region between 1734-1754 nt, which contains two SP1 binding sites, is necessary for transcription origin and HBV replication. We found that the Abf1-binding B3 element in yeast ARS1 can be functionally replaced by the viral Sp1-binding DNA sequence, which activates transcription from the HBV C promoter. Further, yeast RAP1 bound to the viral Sp1 binding sites in vitro. These results suggest that RAP1 binds to the Sp1 binding sites and stimulates yeast DNA replication.

Original languageEnglish (US)
Pages (from-to)737-742
Number of pages6
JournalDNA and Cell Biology
Issue number10
StatePublished - Jan 1 2002


ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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