Heterogeneity of cell surface antigen expression of human small cell lung cancer detected by monoclonal antibodies

S. Fargion, D. Carney, J. Mulshine, S. Rosen, P. Bunn, P. Jewett, F. Cuttitta, A. Gazdar, J. Minna

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Abstract

Using immunohistochemistry, radiobinding, and indirect immunofluorescence assays, seven distinct cell surface antigens, detected by monoclonal antibodies, were analyzed for the degree of homogeneity or heterogeneity of antigen expression on a panel of human small cell lung cancers. The panel included 7 tumors taken directly from patients, 21 established cell lines (9 of which were derived from different metastatic sites of 3 patients), and 33 clonal derivatives of 3 lines. With all assays, considerable heterogeneity of antigen expression between tumors from different patients was observed. In both fresh tumors and in cell lines, as well as in cell lines established from different metastatic sites in an individual patient, we observed intratumor heterogeneity finding antigen positive and negative cells and variation in antigenic density, by immunohistochemistry and indirect immunofluorescence assays. Antigenic expression was not cell cycle dependent. In addition, when cell lines or patient samples expressing antigen positive and antigen negative tumor cells were cloned, heterogeneity of antigenic expression was still present in the clonal lines. This suggests that either the expression of the antigen was not heritable and/or the ability to regenerate antigenic heterogeneity is an intrinsic property of the tumor cells. The heterogeneity of antigen expression on lung cancer cells has significant implications for the use of these and other monoclonal antibodies in the study and therapy of lung cancer.

Original languageEnglish (US)
Pages (from-to)2633-2638
Number of pages6
JournalCancer Research
Volume46
Issue number5
StatePublished - 1986

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Small Cell Lung Carcinoma
Surface Antigens
Monoclonal Antibodies
Antigens
Indirect Fluorescent Antibody Technique
Cell Line
Lung Neoplasms
Immunohistochemistry
Antigenic Variation
Neoplasms
Neoplasm Antigens
Tumor Cell Line
Cell Cycle

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fargion, S., Carney, D., Mulshine, J., Rosen, S., Bunn, P., Jewett, P., ... Minna, J. (1986). Heterogeneity of cell surface antigen expression of human small cell lung cancer detected by monoclonal antibodies. Cancer Research, 46(5), 2633-2638.

Heterogeneity of cell surface antigen expression of human small cell lung cancer detected by monoclonal antibodies. / Fargion, S.; Carney, D.; Mulshine, J.; Rosen, S.; Bunn, P.; Jewett, P.; Cuttitta, F.; Gazdar, A.; Minna, J.

In: Cancer Research, Vol. 46, No. 5, 1986, p. 2633-2638.

Research output: Contribution to journalArticle

Fargion, S, Carney, D, Mulshine, J, Rosen, S, Bunn, P, Jewett, P, Cuttitta, F, Gazdar, A & Minna, J 1986, 'Heterogeneity of cell surface antigen expression of human small cell lung cancer detected by monoclonal antibodies', Cancer Research, vol. 46, no. 5, pp. 2633-2638.
Fargion S, Carney D, Mulshine J, Rosen S, Bunn P, Jewett P et al. Heterogeneity of cell surface antigen expression of human small cell lung cancer detected by monoclonal antibodies. Cancer Research. 1986;46(5):2633-2638.
Fargion, S. ; Carney, D. ; Mulshine, J. ; Rosen, S. ; Bunn, P. ; Jewett, P. ; Cuttitta, F. ; Gazdar, A. ; Minna, J. / Heterogeneity of cell surface antigen expression of human small cell lung cancer detected by monoclonal antibodies. In: Cancer Research. 1986 ; Vol. 46, No. 5. pp. 2633-2638.
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