TY - JOUR
T1 - Heterogeneity of deoxyribonucleic acid molecules isolated from Mycobacterium smegmatis
AU - Norgard, M. V.
AU - Imaeda, T.
PY - 1980/12/1
Y1 - 1980/12/1
N2 - Bulk chromosomal deoxyribonucleic acids (DNAs) of M. smegmatis strains 607+ (wild type) and 607-1 (Str(r)) and orange-red pigmented variants (OR) were separated into two distinct bands (types 1 and 2) by cesium chloride density gradient centrifugation. Thermal denaturation analyses showed that type 1 and 2 DNA fragments of these strains possessed quanine plus cytosine contents averaging 69.2% and 60.8%, respectively. Type 1 and 2 DNAs from all strains tested were recovered in relatively equal quantities upon isolation and were found to have similar molecular weights (3.0x107). Spectrophotometric assay of DNA reassociation showed that homology between any type 1 and 2 DNA fragments was always very low (29 to 33%), even within the same strain. Homologies among type 1 DNAs isolated from any strain were always high (92 to 98%), whereas homologies between type 2 DNA isolated from OR strains and that from their parental strain 607-1 were lower (51 to 55%). Transformation experiments revealed that methionine, leucine, folic acid, and streptomycin markers were found exclusively in type 1 DNA fragments. In addition to the two types of chromosomal DNA, plasmid DNA possessing a molecular weight of about 4x106 was found in strain 607-1.
AB - Bulk chromosomal deoxyribonucleic acids (DNAs) of M. smegmatis strains 607+ (wild type) and 607-1 (Str(r)) and orange-red pigmented variants (OR) were separated into two distinct bands (types 1 and 2) by cesium chloride density gradient centrifugation. Thermal denaturation analyses showed that type 1 and 2 DNA fragments of these strains possessed quanine plus cytosine contents averaging 69.2% and 60.8%, respectively. Type 1 and 2 DNAs from all strains tested were recovered in relatively equal quantities upon isolation and were found to have similar molecular weights (3.0x107). Spectrophotometric assay of DNA reassociation showed that homology between any type 1 and 2 DNA fragments was always very low (29 to 33%), even within the same strain. Homologies among type 1 DNAs isolated from any strain were always high (92 to 98%), whereas homologies between type 2 DNA isolated from OR strains and that from their parental strain 607-1 were lower (51 to 55%). Transformation experiments revealed that methionine, leucine, folic acid, and streptomycin markers were found exclusively in type 1 DNA fragments. In addition to the two types of chromosomal DNA, plasmid DNA possessing a molecular weight of about 4x106 was found in strain 607-1.
UR - http://www.scopus.com/inward/record.url?scp=0019294132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019294132&partnerID=8YFLogxK
M3 - Article
C2 - 6933148
AN - SCOPUS:0019294132
SN - 0021-9193
VL - 144
SP - 766
EP - 771
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 2
ER -