TY - JOUR
T1 - High-resolution mapping of nucleoprotein complexes by site-specific protein-DNA photocrosslinking
T2 - Organization of the human TBP-TFIIA-TFIIB-DNA quaternary complex
AU - Lagrange, Thierry
AU - Kim, Tae Kyung
AU - Orphanides, George
AU - Ebright, Yon W.
AU - Ebricht, Richard H.
AU - Reinberg, Danny
PY - 1996/10/1
Y1 - 1996/10/1
N2 - We have used a novel site-specific protein-DNA photocrosslinking procedure to define the positions of polypeptide chains relative to promoter DNA in binary, ternary, and quaternary complexes containing human TATA- binding protein, human or yeast transcription factor IIA (TFIIA), human transcription factor IIb (TFIIB), and promoter DNA. The results indicate that TFIIA and TFIIB make more extensive interactions with promoter DNA than previously anticipated. TATA-binding protein, TFIIA, and TFIIB surround promoter DNA for two turns of DNA helix and thus may form a 'cylindrical clamp' effectively topologically linked to promoter DNA. Our results have implications for the energetics, DNA-sequence-specificity, and pathway of assembly of eukaryotic transcription complexes.
AB - We have used a novel site-specific protein-DNA photocrosslinking procedure to define the positions of polypeptide chains relative to promoter DNA in binary, ternary, and quaternary complexes containing human TATA- binding protein, human or yeast transcription factor IIA (TFIIA), human transcription factor IIb (TFIIB), and promoter DNA. The results indicate that TFIIA and TFIIB make more extensive interactions with promoter DNA than previously anticipated. TATA-binding protein, TFIIA, and TFIIB surround promoter DNA for two turns of DNA helix and thus may form a 'cylindrical clamp' effectively topologically linked to promoter DNA. Our results have implications for the energetics, DNA-sequence-specificity, and pathway of assembly of eukaryotic transcription complexes.
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U2 - 10.1073/pnas.93.20.10620
DO - 10.1073/pnas.93.20.10620
M3 - Article
C2 - 8855228
AN - SCOPUS:0029785839
SN - 0027-8424
VL - 93
SP - 10620
EP - 10625
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -