High-throughput quantitative bioluminescence imaging for assessing tumor burden

Angelina Contero, Edmond Richer, Ana Gondim, Ralph P. Mason

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

Bioluminescence imaging (BLI) has emerged during the past 5 years as the preeminent method for rapid, cheap, facile screening of tumor growth and spread in mice. Both subcutaneous and orthotopic tumor models are readily observed with high sensitivity and reproducibility. User-friendly commercial instruments exist and, increasingly, luciferase-expressing tumor cells are available in academic institutions or commercially. There is an increasing literature on routine use of BLI for assessing chemotherapeutic efficacy, drug combinations, dosing, and timing. In addition, BLI may be applied to more sophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages37-45
Number of pages9
Volume574
ISBN (Print)9781603273206
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume574
ISSN (Print)10643745

Keywords

  • bioluminescence
  • charged-coupled device cameras (CCD)
  • IGOR Pro
  • Luciferase
  • luciferin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Contero, A., Richer, E., Gondim, A., & Mason, R. P. (2009). High-throughput quantitative bioluminescence imaging for assessing tumor burden. In Methods in Molecular Biology (Vol. 574, pp. 37-45). (Methods in Molecular Biology; Vol. 574). Humana Press Inc.. https://doi.org/10.1007/978-1-60327-321-3_4