High-throughput quantitative bioluminescence imaging for assessing tumor burden

Angelina Contero, Edmond Richer, Ana Gondim, Ralph P. Mason

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Bioluminescence imaging (BLI) has emerged during the past 5 years as the preeminent method for rapid, cheap, facile screening of tumor growth and spread in mice. Both subcutaneous and orthotopic tumor models are readily observed with high sensitivity and reproducibility. User-friendly commercial instruments exist and, increasingly, luciferase-expressing tumor cells are available in academic institutions or commercially. There is an increasing literature on routine use of BLI for assessing chemotherapeutic efficacy, drug combinations, dosing, and timing. In addition, BLI may be applied to more sophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages37-45
Number of pages9
Volume574
ISBN (Print)9781603273206
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume574
ISSN (Print)10643745

Fingerprint

Tumor Burden
Neoplasms
Drug Combinations
Luciferases
Molecular Biology
Research Personnel
Growth

Keywords

  • bioluminescence
  • charged-coupled device cameras (CCD)
  • IGOR Pro
  • Luciferase
  • luciferin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Contero, A., Richer, E., Gondim, A., & Mason, R. P. (2009). High-throughput quantitative bioluminescence imaging for assessing tumor burden. In Methods in Molecular Biology (Vol. 574, pp. 37-45). (Methods in Molecular Biology; Vol. 574). Humana Press Inc.. https://doi.org/10.1007/978-1-60327-321-3_4

High-throughput quantitative bioluminescence imaging for assessing tumor burden. / Contero, Angelina; Richer, Edmond; Gondim, Ana; Mason, Ralph P.

Methods in Molecular Biology. Vol. 574 Humana Press Inc., 2009. p. 37-45 (Methods in Molecular Biology; Vol. 574).

Research output: Chapter in Book/Report/Conference proceedingChapter

Contero, A, Richer, E, Gondim, A & Mason, RP 2009, High-throughput quantitative bioluminescence imaging for assessing tumor burden. in Methods in Molecular Biology. vol. 574, Methods in Molecular Biology, vol. 574, Humana Press Inc., pp. 37-45. https://doi.org/10.1007/978-1-60327-321-3_4
Contero A, Richer E, Gondim A, Mason RP. High-throughput quantitative bioluminescence imaging for assessing tumor burden. In Methods in Molecular Biology. Vol. 574. Humana Press Inc. 2009. p. 37-45. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-321-3_4
Contero, Angelina ; Richer, Edmond ; Gondim, Ana ; Mason, Ralph P. / High-throughput quantitative bioluminescence imaging for assessing tumor burden. Methods in Molecular Biology. Vol. 574 Humana Press Inc., 2009. pp. 37-45 (Methods in Molecular Biology).
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