Histological evaluation of 3D MRI-guided transurethral ultrasound therapy in the prostate

Siddharth Vedula, Aaron Boyes, Rajiv Chopra, Michael Bronskill

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Previous work from our group has shown that transurethral ultrasound therapy, with a single ultrasound transducer guided by temperature feedback from a single MRI plane (slice), can be used to treat a targeted region accurately in the prostate gland. We have extended this approach to a larger, 3D, targeted volume within the prostate, using a multi-element transducer controlled concurrently by temperature feedback from multiple imaging planes. Animals were placed supine in a 1.5 T clinical MRI, and the transurethral heating device was positioned with image guidance. A four-element transducer (each element was 5 mm long, operating at ∼8 MHz) was rotated to treat a targeted volume around the device. Temperature maps transverse to each element were acquired during heating and used to control the acoustic power of each element and the rate of rotation of the device. T2-weighted and contrast-enhanced (CE) MR images were obtained pre- and post-heating. Following the treatment, prostates were removed and fixed, axially sliced, stained with H&E, and digitally imaged at high-resolution to outline boundaries of cell death. Slice alignment and image registration techniques were developed to enable quantitative comparison of the axial MRI images and matching histological sections. Prostate sections showed clear regions of coagulative necrosis, extending ∼20 mm along the urethra, which correlated well with CE MRI data and transducer length. After registration, the outer border of coagulative necrosis on H&E conformed well to the target isotherm, similar to results from our previous (single element) acute studies. These results confirm that our previous analysis techniques for a single transducer can be extended to multiple elements, and that a large volumetric ablation of the prostate gland is feasible with a high degree of accuracy.

Original languageEnglish (US)
Title of host publication9th International Symposium on Therapeutic Ultrasound - ISTU 2009
Pages224-228
Number of pages5
DOIs
StatePublished - Mar 30 2010
Event9th International Symposium on Therapeutic Ultrasound, ISTU 2009 - Aix-en-Provence, France
Duration: Sep 24 2009Sep 26 2009

Publication series

NameAIP Conference Proceedings
Volume1215
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other9th International Symposium on Therapeutic Ultrasound, ISTU 2009
CountryFrance
CityAix-en-Provence
Period9/24/099/26/09

Keywords

  • Histology
  • Image registration
  • Prostate
  • Thermal damage
  • Ultrasound therapy

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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  • Cite this

    Vedula, S., Boyes, A., Chopra, R., & Bronskill, M. (2010). Histological evaluation of 3D MRI-guided transurethral ultrasound therapy in the prostate. In 9th International Symposium on Therapeutic Ultrasound - ISTU 2009 (pp. 224-228). (AIP Conference Proceedings; Vol. 1215). https://doi.org/10.1063/1.3367147