Histone H3K56 acetylation is required for quelling-induced small RNA production through its role in homologous recombination

Zhenyu Zhang, Qiuying Yang, Guangyan Sun, She Chen, Qun He, Shaojie Li, Yi Liu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Quelling and DNA damage-induced small RNA (qiRNA) production are RNA interference (RNAi)-related phenomenon from repetitive genomic loci in Neurospora. We have recently proposed that homologous recombination from repetitive DNA loci allows the RNAi pathway to recognize repetitive DNA to produce small RNA. However, the mechanistic detail of this pathway remains largely unclear. By systematically screening the Neurospora knock-out library, we identified RTT109 as a novel component required for small RNA production. RTT109 is a histone acetyltransferase for histone H3 lysine 56 (H3K56) and H3K56 acetylation is essential for the small RNA biogenesis pathway. Furthermore, we showed that RTT109 is required for homologous recombination and H3K56Ac is enriched around double strand break, which overlaps with RAD51 binding. Taken together, our results suggest that H3K56 acetylation is required for small RNA production through its role in homologous recombination.

Original languageEnglish (US)
Pages (from-to)9365-9371
Number of pages7
JournalJournal of Biological Chemistry
Volume289
Issue number13
DOIs
StatePublished - Mar 30 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Histone H3K56 acetylation is required for quelling-induced small RNA production through its role in homologous recombination'. Together they form a unique fingerprint.

Cite this