TY - JOUR
T1 - Histone H3K56 acetylation is required for quelling-induced small RNA production through its role in homologous recombination
AU - Zhang, Zhenyu
AU - Yang, Qiuying
AU - Sun, Guangyan
AU - Chen, She
AU - He, Qun
AU - Li, Shaojie
AU - Liu, Yi
PY - 2014/3/30
Y1 - 2014/3/30
N2 - Quelling and DNA damage-induced small RNA (qiRNA) production are RNA interference (RNAi)-related phenomenon from repetitive genomic loci in Neurospora. We have recently proposed that homologous recombination from repetitive DNA loci allows the RNAi pathway to recognize repetitive DNA to produce small RNA. However, the mechanistic detail of this pathway remains largely unclear. By systematically screening the Neurospora knock-out library, we identified RTT109 as a novel component required for small RNA production. RTT109 is a histone acetyltransferase for histone H3 lysine 56 (H3K56) and H3K56 acetylation is essential for the small RNA biogenesis pathway. Furthermore, we showed that RTT109 is required for homologous recombination and H3K56Ac is enriched around double strand break, which overlaps with RAD51 binding. Taken together, our results suggest that H3K56 acetylation is required for small RNA production through its role in homologous recombination.
AB - Quelling and DNA damage-induced small RNA (qiRNA) production are RNA interference (RNAi)-related phenomenon from repetitive genomic loci in Neurospora. We have recently proposed that homologous recombination from repetitive DNA loci allows the RNAi pathway to recognize repetitive DNA to produce small RNA. However, the mechanistic detail of this pathway remains largely unclear. By systematically screening the Neurospora knock-out library, we identified RTT109 as a novel component required for small RNA production. RTT109 is a histone acetyltransferase for histone H3 lysine 56 (H3K56) and H3K56 acetylation is essential for the small RNA biogenesis pathway. Furthermore, we showed that RTT109 is required for homologous recombination and H3K56Ac is enriched around double strand break, which overlaps with RAD51 binding. Taken together, our results suggest that H3K56 acetylation is required for small RNA production through its role in homologous recombination.
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U2 - 10.1074/jbc.M113.528521
DO - 10.1074/jbc.M113.528521
M3 - Article
C2 - 24554705
AN - SCOPUS:84897422815
SN - 0021-9258
VL - 289
SP - 9365
EP - 9371
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 13
ER -