Histone variant H3.3 is an essential maternal factor for oocyte reprogramming

Duancheng Wen, Laura A. Banaszynski, Ying Liu, Fuqiang Geng, Kyung Min Noh, Jenny Xiang, Olivier Elemento, Zev Rosenwaks, C. David Allis, Shahin Rafii

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Mature oocyte cytoplasm can reprogram somatic cell nuclei to the pluripotent state through a series of sequential events including protein exchange between the donor nucleus and ooplasm, chromatin remodeling, and pluripotency gene reactivation. Maternal factors that are responsible for this reprogramming process remain largely unidentified. Here, we demonstrate that knockdown of histone variant H3.3 in mouse oocytes results in compromised reprogramming and down-regulation of key pluripotency genes; and this compromised reprogramming for developmental potentials and transcription of pluripotency genes can be rescued by injecting exogenous H3.3 mRNA, but not H3.2 mRNA, into oocytes in somatic cell nuclear transfer embryos. We show that maternal H3.3, and not H3.3 in the donor nucleus, is essential for successful reprogramming of somatic cell nucleus into the pluripotent state. Furthermore, H3.3 is involved in this reprogramming process by remodeling the donor nuclear chromatin through replacement of donor nucleus-derived H3 with de novo synthesized maternal H3.3 protein. Our study shows that H3.3 is a crucial maternal factor for oocyte reprogramming and provides a practical model to directly dissect the oocyte for its reprogramming capacity.

Original languageEnglish (US)
Pages (from-to)7325-7330
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number20
DOIs
StatePublished - May 20 2014

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Histones
Oocytes
Mothers
Cell Nucleus
Genes
Messenger RNA
Chromatin Assembly and Disassembly
Embryo Transfer
Chromatin
Cytoplasm
Proteins
Down-Regulation

ASJC Scopus subject areas

  • General

Cite this

Histone variant H3.3 is an essential maternal factor for oocyte reprogramming. / Wen, Duancheng; Banaszynski, Laura A.; Liu, Ying; Geng, Fuqiang; Noh, Kyung Min; Xiang, Jenny; Elemento, Olivier; Rosenwaks, Zev; David Allis, C.; Rafii, Shahin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 20, 20.05.2014, p. 7325-7330.

Research output: Contribution to journalArticle

Wen, D, Banaszynski, LA, Liu, Y, Geng, F, Noh, KM, Xiang, J, Elemento, O, Rosenwaks, Z, David Allis, C & Rafii, S 2014, 'Histone variant H3.3 is an essential maternal factor for oocyte reprogramming', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 20, pp. 7325-7330. https://doi.org/10.1073/pnas.1406389111
Wen, Duancheng ; Banaszynski, Laura A. ; Liu, Ying ; Geng, Fuqiang ; Noh, Kyung Min ; Xiang, Jenny ; Elemento, Olivier ; Rosenwaks, Zev ; David Allis, C. ; Rafii, Shahin. / Histone variant H3.3 is an essential maternal factor for oocyte reprogramming. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 20. pp. 7325-7330.
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