Homer regulation of native plasma membrane calcium channels in A431 cells

Alexey Shalygin, Maria Ryazantseva, Lyuba Glushankova, Galina N. Mozhayeva, Ilya Bezprozvanny, Elena Kaznacheyeva

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Homers are adapter proteins that play a significant role in the organization of calcium signaling protein complexes. Previous functional studies linked Homer proteins to calcium influx in nonexcitable cells. These studies utilized calcium imaging or whole-cell current recordings. Because of limited resolution of these methods, an identity of Homer-modulated ion channels remained unclear. There are several types of plasma membrane calcium influx channels in A431 cells. In the present study, we demonstrated that Homer dissociation resulted in specific activation of Imin channels but not of Imax channels in inside-out patches taken from A431 cells. In contrast, inositol 1,4,5-trisphosphate activated both Imin and Imax channels in inside-out patches. Short (1a) and long (1c) forms of Homer had different effects on Imin channel activity. Homer 1a but not Homer 1c activated Imin in the patches. This study indicates that Imin channels are specifically regulated by Homer proteins in A431 cells.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalCell Calcium
Volume48
Issue number4
DOIs
StatePublished - Oct 2010

Keywords

  • A431
  • Adaptor proteins
  • Calcium signalling
  • Homer
  • IP3R
  • Patch clamp
  • SOC

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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