Homer regulation of native plasma membrane calcium channels in A431 cells

Alexey Shalygin, Maria Ryazantseva, Lyuba Glushankova, Galina N. Mozhayeva, Ilya Bezprozvanny, Elena Kaznacheyeva

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Homers are adapter proteins that play a significant role in the organization of calcium signaling protein complexes. Previous functional studies linked Homer proteins to calcium influx in nonexcitable cells. These studies utilized calcium imaging or whole-cell current recordings. Because of limited resolution of these methods, an identity of Homer-modulated ion channels remained unclear. There are several types of plasma membrane calcium influx channels in A431 cells. In the present study, we demonstrated that Homer dissociation resulted in specific activation of Imin channels but not of Imax channels in inside-out patches taken from A431 cells. In contrast, inositol 1,4,5-trisphosphate activated both Imin and Imax channels in inside-out patches. Short (1a) and long (1c) forms of Homer had different effects on Imin channel activity. Homer 1a but not Homer 1c activated Imin in the patches. This study indicates that Imin channels are specifically regulated by Homer proteins in A431 cells.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalCell Calcium
Volume48
Issue number4
DOIs
StatePublished - Oct 2010

Fingerprint

Calcium Channels
Ion Channels
Cell Membrane
Calcium
Inositol 1,4,5-Trisphosphate
Calcium Signaling
Patch-Clamp Techniques
Homer Scaffolding Proteins
Proteins

Keywords

  • A431
  • Adaptor proteins
  • Calcium signalling
  • Homer
  • IP3R
  • Patch clamp
  • SOC

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Shalygin, A., Ryazantseva, M., Glushankova, L., Mozhayeva, G. N., Bezprozvanny, I., & Kaznacheyeva, E. (2010). Homer regulation of native plasma membrane calcium channels in A431 cells. Cell Calcium, 48(4), 209-214. https://doi.org/10.1016/j.ceca.2010.09.002

Homer regulation of native plasma membrane calcium channels in A431 cells. / Shalygin, Alexey; Ryazantseva, Maria; Glushankova, Lyuba; Mozhayeva, Galina N.; Bezprozvanny, Ilya; Kaznacheyeva, Elena.

In: Cell Calcium, Vol. 48, No. 4, 10.2010, p. 209-214.

Research output: Contribution to journalArticle

Shalygin, A, Ryazantseva, M, Glushankova, L, Mozhayeva, GN, Bezprozvanny, I & Kaznacheyeva, E 2010, 'Homer regulation of native plasma membrane calcium channels in A431 cells', Cell Calcium, vol. 48, no. 4, pp. 209-214. https://doi.org/10.1016/j.ceca.2010.09.002
Shalygin A, Ryazantseva M, Glushankova L, Mozhayeva GN, Bezprozvanny I, Kaznacheyeva E. Homer regulation of native plasma membrane calcium channels in A431 cells. Cell Calcium. 2010 Oct;48(4):209-214. https://doi.org/10.1016/j.ceca.2010.09.002
Shalygin, Alexey ; Ryazantseva, Maria ; Glushankova, Lyuba ; Mozhayeva, Galina N. ; Bezprozvanny, Ilya ; Kaznacheyeva, Elena. / Homer regulation of native plasma membrane calcium channels in A431 cells. In: Cell Calcium. 2010 ; Vol. 48, No. 4. pp. 209-214.
@article{3707f32e73d04a25a1dff882516f90d9,
title = "Homer regulation of native plasma membrane calcium channels in A431 cells",
abstract = "Homers are adapter proteins that play a significant role in the organization of calcium signaling protein complexes. Previous functional studies linked Homer proteins to calcium influx in nonexcitable cells. These studies utilized calcium imaging or whole-cell current recordings. Because of limited resolution of these methods, an identity of Homer-modulated ion channels remained unclear. There are several types of plasma membrane calcium influx channels in A431 cells. In the present study, we demonstrated that Homer dissociation resulted in specific activation of Imin channels but not of Imax channels in inside-out patches taken from A431 cells. In contrast, inositol 1,4,5-trisphosphate activated both Imin and Imax channels in inside-out patches. Short (1a) and long (1c) forms of Homer had different effects on Imin channel activity. Homer 1a but not Homer 1c activated Imin in the patches. This study indicates that Imin channels are specifically regulated by Homer proteins in A431 cells.",
keywords = "A431, Adaptor proteins, Calcium signalling, Homer, IP3R, Patch clamp, SOC",
author = "Alexey Shalygin and Maria Ryazantseva and Lyuba Glushankova and Mozhayeva, {Galina N.} and Ilya Bezprozvanny and Elena Kaznacheyeva",
year = "2010",
month = "10",
doi = "10.1016/j.ceca.2010.09.002",
language = "English (US)",
volume = "48",
pages = "209--214",
journal = "Cell Calcium",
issn = "0143-4160",
publisher = "Churchill Livingstone",
number = "4",

}

TY - JOUR

T1 - Homer regulation of native plasma membrane calcium channels in A431 cells

AU - Shalygin, Alexey

AU - Ryazantseva, Maria

AU - Glushankova, Lyuba

AU - Mozhayeva, Galina N.

AU - Bezprozvanny, Ilya

AU - Kaznacheyeva, Elena

PY - 2010/10

Y1 - 2010/10

N2 - Homers are adapter proteins that play a significant role in the organization of calcium signaling protein complexes. Previous functional studies linked Homer proteins to calcium influx in nonexcitable cells. These studies utilized calcium imaging or whole-cell current recordings. Because of limited resolution of these methods, an identity of Homer-modulated ion channels remained unclear. There are several types of plasma membrane calcium influx channels in A431 cells. In the present study, we demonstrated that Homer dissociation resulted in specific activation of Imin channels but not of Imax channels in inside-out patches taken from A431 cells. In contrast, inositol 1,4,5-trisphosphate activated both Imin and Imax channels in inside-out patches. Short (1a) and long (1c) forms of Homer had different effects on Imin channel activity. Homer 1a but not Homer 1c activated Imin in the patches. This study indicates that Imin channels are specifically regulated by Homer proteins in A431 cells.

AB - Homers are adapter proteins that play a significant role in the organization of calcium signaling protein complexes. Previous functional studies linked Homer proteins to calcium influx in nonexcitable cells. These studies utilized calcium imaging or whole-cell current recordings. Because of limited resolution of these methods, an identity of Homer-modulated ion channels remained unclear. There are several types of plasma membrane calcium influx channels in A431 cells. In the present study, we demonstrated that Homer dissociation resulted in specific activation of Imin channels but not of Imax channels in inside-out patches taken from A431 cells. In contrast, inositol 1,4,5-trisphosphate activated both Imin and Imax channels in inside-out patches. Short (1a) and long (1c) forms of Homer had different effects on Imin channel activity. Homer 1a but not Homer 1c activated Imin in the patches. This study indicates that Imin channels are specifically regulated by Homer proteins in A431 cells.

KW - A431

KW - Adaptor proteins

KW - Calcium signalling

KW - Homer

KW - IP3R

KW - Patch clamp

KW - SOC

UR - http://www.scopus.com/inward/record.url?scp=77958194486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958194486&partnerID=8YFLogxK

U2 - 10.1016/j.ceca.2010.09.002

DO - 10.1016/j.ceca.2010.09.002

M3 - Article

C2 - 20926133

AN - SCOPUS:77958194486

VL - 48

SP - 209

EP - 214

JO - Cell Calcium

JF - Cell Calcium

SN - 0143-4160

IS - 4

ER -