Host-derived nitrate boosts growth of E. coli in the inflamed gut

Sebastian E. Winter, Maria G. Winter, Mariana N. Xavier, Parameth Thiennimitr, Victor Poon, A. Marijke Keestra, Richard C. Laughlin, Gabriel Gomez, Jing Wu, Sara D. Lawhon, Ina E. Popova, Sanjai J. Parikh, L. Garry Adams, Renée M. Tsolis, Valley J. Stewart, Andreas J. Bäumler

Research output: Contribution to journalArticle

344 Citations (Scopus)

Abstract

Changes in the microbial community structure are observed in individuals with intestinal inflammatory disorders. These changes are often characterized by a depletion of obligate anaerobic bacteria, whereas the relative abundance of facultative anaerobic Enterobacteriaceae increases. The mechanisms by which the host response shapes the microbial community structure, however, remain unknown. We show that nitrate generated as a by-product of the inflammatory response conferred a growth advantage to the commensal bacterium Escherichia coli in the large intestine of mice. Mice deficient in inducible nitric oxide synthase did not support the growth of E. coli by nitrate respiration, suggesting that the nitrate generated during inflammation was host-derived. Thus, the inflammatory host response selectively enhances the growth of commensal Enterobacteriaceae by generating electron acceptors for anaerobic respiration.

Original languageEnglish (US)
Pages (from-to)708-711
Number of pages4
JournalScience
Volume339
Issue number6120
DOIs
StatePublished - Feb 8 2013

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Nitrates
Enterobacteriaceae
Escherichia coli
Respiration
Growth
Anaerobic Bacteria
Large Intestine
Nitric Oxide Synthase Type II
Electrons
Inflammation
Bacteria

ASJC Scopus subject areas

  • General

Cite this

Winter, S. E., Winter, M. G., Xavier, M. N., Thiennimitr, P., Poon, V., Keestra, A. M., ... Bäumler, A. J. (2013). Host-derived nitrate boosts growth of E. coli in the inflamed gut. Science, 339(6120), 708-711. https://doi.org/10.1126/science.1232467

Host-derived nitrate boosts growth of E. coli in the inflamed gut. / Winter, Sebastian E.; Winter, Maria G.; Xavier, Mariana N.; Thiennimitr, Parameth; Poon, Victor; Keestra, A. Marijke; Laughlin, Richard C.; Gomez, Gabriel; Wu, Jing; Lawhon, Sara D.; Popova, Ina E.; Parikh, Sanjai J.; Adams, L. Garry; Tsolis, Renée M.; Stewart, Valley J.; Bäumler, Andreas J.

In: Science, Vol. 339, No. 6120, 08.02.2013, p. 708-711.

Research output: Contribution to journalArticle

Winter, SE, Winter, MG, Xavier, MN, Thiennimitr, P, Poon, V, Keestra, AM, Laughlin, RC, Gomez, G, Wu, J, Lawhon, SD, Popova, IE, Parikh, SJ, Adams, LG, Tsolis, RM, Stewart, VJ & Bäumler, AJ 2013, 'Host-derived nitrate boosts growth of E. coli in the inflamed gut', Science, vol. 339, no. 6120, pp. 708-711. https://doi.org/10.1126/science.1232467
Winter SE, Winter MG, Xavier MN, Thiennimitr P, Poon V, Keestra AM et al. Host-derived nitrate boosts growth of E. coli in the inflamed gut. Science. 2013 Feb 8;339(6120):708-711. https://doi.org/10.1126/science.1232467
Winter, Sebastian E. ; Winter, Maria G. ; Xavier, Mariana N. ; Thiennimitr, Parameth ; Poon, Victor ; Keestra, A. Marijke ; Laughlin, Richard C. ; Gomez, Gabriel ; Wu, Jing ; Lawhon, Sara D. ; Popova, Ina E. ; Parikh, Sanjai J. ; Adams, L. Garry ; Tsolis, Renée M. ; Stewart, Valley J. ; Bäumler, Andreas J. / Host-derived nitrate boosts growth of E. coli in the inflamed gut. In: Science. 2013 ; Vol. 339, No. 6120. pp. 708-711.
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