How Tlg2p/syntaxin 16 'snares' Vps45

Irina Dulubova, Tomohiro Yamaguchi, Yan Gao, Sang Won Min, Iryna Huryeva, Thomas C. Südhof, Jose Rizo-Rey

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Soluble N-ethylmaleimide sensitive factor-attachment protein receptors (SNAREs) and Sec1p/Munc18 homologs (SM proteins) play key roles in intracellular membrane fusion. The SNAREs form tight four-helix bundles (core complexes) that bring the membranes together, but it is unclear how this activity is coupled to SM protein function. Studies of the yeast trans-Golgi network (TGN)/endosomal SNARE complex, which includes the syntaxin-like SNARE Tlg2p, have suggested that its assembly requires activation by binding of the SM protein Vps45p to the cytoplasmic region of Tlg2p folded into a closed conformation. Nuclear magnetic resonance and biochemical experiments now show that Tlg2p and Pep12p, a late-endosomal syntaxin that interacts functionally but not directly with Vps45p, have a domain structure characteristic of syntaxins but do not adopt a closed conformation. Tlg2p binds tightly to Vps45p via a short N-terminal peptide motif that is absent in Pep12p. The Tlg2p/Vps45p binding mode is shared by the mammalian syntaxin 16, confirming that it is a Tlg2p homolog, and resembles the mode of interaction between the SM protein Sly1p and the syntaxins Ufe1p and Sed5p. Thus, this mechanism represents the most widespread mode of coupling between syntaxins and SM proteins.

Original languageEnglish (US)
Pages (from-to)3620-3631
Number of pages12
JournalEMBO Journal
Volume21
Issue number14
DOIs
StatePublished - Jul 15 2002

Fingerprint

Syntaxin 16
Qa-SNARE Proteins
Proteins
trans-Golgi Network
SNARE Proteins
Intracellular Membranes
Membrane Fusion
Conformations
Membranes
Carrier Proteins
Magnetic Resonance Spectroscopy
Yeasts
Peptides
Yeast
Fusion reactions

Keywords

  • SM proteins
  • SNAREs
  • Syntaxins
  • Tlg2p
  • Vps45p

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Dulubova, I., Yamaguchi, T., Gao, Y., Min, S. W., Huryeva, I., Südhof, T. C., & Rizo-Rey, J. (2002). How Tlg2p/syntaxin 16 'snares' Vps45. EMBO Journal, 21(14), 3620-3631. https://doi.org/10.1093/emboj/cdf381

How Tlg2p/syntaxin 16 'snares' Vps45. / Dulubova, Irina; Yamaguchi, Tomohiro; Gao, Yan; Min, Sang Won; Huryeva, Iryna; Südhof, Thomas C.; Rizo-Rey, Jose.

In: EMBO Journal, Vol. 21, No. 14, 15.07.2002, p. 3620-3631.

Research output: Contribution to journalArticle

Dulubova, I, Yamaguchi, T, Gao, Y, Min, SW, Huryeva, I, Südhof, TC & Rizo-Rey, J 2002, 'How Tlg2p/syntaxin 16 'snares' Vps45', EMBO Journal, vol. 21, no. 14, pp. 3620-3631. https://doi.org/10.1093/emboj/cdf381
Dulubova I, Yamaguchi T, Gao Y, Min SW, Huryeva I, Südhof TC et al. How Tlg2p/syntaxin 16 'snares' Vps45. EMBO Journal. 2002 Jul 15;21(14):3620-3631. https://doi.org/10.1093/emboj/cdf381
Dulubova, Irina ; Yamaguchi, Tomohiro ; Gao, Yan ; Min, Sang Won ; Huryeva, Iryna ; Südhof, Thomas C. ; Rizo-Rey, Jose. / How Tlg2p/syntaxin 16 'snares' Vps45. In: EMBO Journal. 2002 ; Vol. 21, No. 14. pp. 3620-3631.
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