HP1α guides neuronal fate by timing E2F-targeted genes silencing during terminal differentiation

Irina Panteleeva, Stéphanie Boutillier, Violaine See, Dave G. Spiller, Caroline Rouaux, Geneviève Almouzni, Delphine Bailly, Christèle Maison, Helen C. Lai, Jean Philippe Loeffler, Anne Laurence Boutillier

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A critical step of neuronal terminal differentiation is the permanent withdrawal from the cell cycle that requires the silencing of genes that drive mitosis. Here, we describe that the α isoform of the heterochromatin protein 1 (HP1) protein family exerts such silencing on several E2F-targeted genes. Among the different isoforms, HP1α levels progressively increase throughout differentiation and take over HP1γ binding on E2F sites in mature neurons. When overexpressed, only HP1α is able to ensure a timed repression of E2F genes. Specific inhibition of HP1α expression drives neuronal progenitors either towards death or cell cycle progression, yet preventing the expression of the neuronal marker microtubule-associated protein 2. Furthermore, we provide evidence that this mechanism occurs in cerebellar granule neurons in vivo, during the postnatal development of the cerebellum. Finally, our results suggest that E2F-targeted genes are packaged into higher-order chromatin structures in mature neurons relative to neuroblasts, likely reflecting a transition from a 'repressed' versus 'silenced' status of these genes. Together, these data present new epigenetic regulations orchestrated by HP1 isoforms, critical for permanent cell cycle exit during neuronal differentiation.

Original languageEnglish (US)
Pages (from-to)3616-3628
Number of pages13
JournalEMBO Journal
Volume26
Issue number15
DOIs
StatePublished - Aug 8 2007

Fingerprint

Gene Silencing
Genes
Neurons
Cell Cycle
Protein Isoforms
Cells
Microtubule-Associated Proteins
Mitosis
Protein Binding
Epigenomics
Cerebellum
Chromatin
heterochromatin-specific nonhistone chromosomal protein HP-1
Proteins

Keywords

  • E2F
  • Heterochromatin
  • HP1́
  • Neuronal terminal differentiation
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Panteleeva, I., Boutillier, S., See, V., Spiller, D. G., Rouaux, C., Almouzni, G., ... Boutillier, A. L. (2007). HP1α guides neuronal fate by timing E2F-targeted genes silencing during terminal differentiation. EMBO Journal, 26(15), 3616-3628. https://doi.org/10.1038/sj.emboj.7601789

HP1α guides neuronal fate by timing E2F-targeted genes silencing during terminal differentiation. / Panteleeva, Irina; Boutillier, Stéphanie; See, Violaine; Spiller, Dave G.; Rouaux, Caroline; Almouzni, Geneviève; Bailly, Delphine; Maison, Christèle; Lai, Helen C.; Loeffler, Jean Philippe; Boutillier, Anne Laurence.

In: EMBO Journal, Vol. 26, No. 15, 08.08.2007, p. 3616-3628.

Research output: Contribution to journalArticle

Panteleeva, I, Boutillier, S, See, V, Spiller, DG, Rouaux, C, Almouzni, G, Bailly, D, Maison, C, Lai, HC, Loeffler, JP & Boutillier, AL 2007, 'HP1α guides neuronal fate by timing E2F-targeted genes silencing during terminal differentiation', EMBO Journal, vol. 26, no. 15, pp. 3616-3628. https://doi.org/10.1038/sj.emboj.7601789
Panteleeva I, Boutillier S, See V, Spiller DG, Rouaux C, Almouzni G et al. HP1α guides neuronal fate by timing E2F-targeted genes silencing during terminal differentiation. EMBO Journal. 2007 Aug 8;26(15):3616-3628. https://doi.org/10.1038/sj.emboj.7601789
Panteleeva, Irina ; Boutillier, Stéphanie ; See, Violaine ; Spiller, Dave G. ; Rouaux, Caroline ; Almouzni, Geneviève ; Bailly, Delphine ; Maison, Christèle ; Lai, Helen C. ; Loeffler, Jean Philippe ; Boutillier, Anne Laurence. / HP1α guides neuronal fate by timing E2F-targeted genes silencing during terminal differentiation. In: EMBO Journal. 2007 ; Vol. 26, No. 15. pp. 3616-3628.
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